Rabbit Anti-MFN2 Recombinant Antibody (clone R56-8V-9) (CAT#: VS3-FY2117)

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse and Rat MFN2

• Species Reactivity
• Human, Mouse, Rat

• Clone
• R56-8V-9

• Applications
• Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence

• Conjugate
• Unconjugated

• MW
• The calculated weight of the target protein is approximately 86 kDa, while the observed molecular weight stands at 80kDa.

• Related Disease
• Charcot-Marie-Tooth disease type 2A2, and hereditary motor and sensory neuropathy VI

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• PBS (pH 7.4), 150 mM NaCl, 50% glycerol

• Preservative
• 0.02% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunohistochemistry: 1/100-1/200
  Immunofluorescence: 1/50-1/200

• Alternative Names
• Mitofusin 2; Transmembrane GTPase MFN2; CPRP1; Mitochondrial Assembly Regulatory Factor; Hyperplasia Suppressor; Mitofusin-2; EC 3.6.5.-; KIAA0214; EC 3.6.5

• Gene ID
• 9927

• UniProt ID
• O95140

• Sequence Similarities
• Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. Mitofusin subfamily.

• Cellular Localization
• Membrane, Mitochondrion, Mitochondrion outer membrane

• Post Translation Modifications
• Phosphorylated by PINK1. (O95140-MFN2_HUMAN)
  Ubiquitinated by non-degradative ubiquitin by PRKN, promoting mitochondrial fusion; deubiquitination by USP30 inhibits mitochondrial fusion (PubMed:23620051).
  Ubiquitinated by HUWE1 when dietary stearate (C18:0) levels are low; ubiquitination inhibits mitochondrial fusion (PubMed:26214738, 30217973).
  (O95140-MFN2_HUMAN)
  Ubiquitination at Lys406, Lys416, and Lys420 (NX_O95140 [NX_O95140-1])
  Modification sites at PhosphoSitePlus (O95140)

• Protein Refseq
• XP_005263600.1; XP_005263602.1; XP_005263605.1; XP_047292105.1; XP_047292110.1

• Function
• Plays an important role in the regulation of vascular smooth muscle cell proliferation (By similarity).
  Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy).
  Is required for PRKN recruitment to dysfunctional mitochondria.
  Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress (By similarity).
  Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions (By similarity).

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody