Mouse Anti-MHC I Recombinant Antibody (clone OX27) (CAT#: FAMAB-1321CQ)

Figure 1 MHC class I Abs cause ALI in a 2-event in vivo model.

Rats were injected with saline (NS) or LPS 2 hours prior to the infusion of 0.15 mg/kg OX27. After completion of the experimental protocol, the rats were humanely killed, and the lungs were snap-frozen and fixed, embedded in paraffin, and sectioned. All images were shot at 40×, and the sections were stained with H&E.

Kelher, M. R., Masuno, T., Moore, E. E., Damle, S., Meng, X., Song, Y., ... & Gamboni-Robertson, F. (2009). Plasma from stored packed red blood cells and MHC class I antibodies causes acute lung injury in a 2-event in vivo rat model. Blood, The Journal of the American Society of Hematology, 113(9), 2079-2087.

Figure 2 MHC class I Abs cause ALI in a 2-event in vivo model.

Rats were injected with saline (NS) or LPS 2 hours prior to the infusion of 0.15 mg/kg OX27. After completion of the experimental protocol, the rats were humanely killed, and the lungs were snap-frozen and fixed, embedded in paraffin, and sectioned. All images were shot at 40×, and the sections were stained with H&E to localize rat PMNs (red) and to demonstrate the pulmonary parenchyma by staining the membranes green (WGA linked to Cy-3) and the nuclei blue (bis-benzamide).

Kelher, M. R., Masuno, T., Moore, E. E., Damle, S., Meng, X., Song, Y., ... & Gamboni-Robertson, F. (2009). Plasma from stored packed red blood cells and MHC class I antibodies causes acute lung injury in a 2-event in vivo rat model. Blood, The Journal of the American Society of Hematology, 113(9), 2079-2087.

Figure 3 Rat MHC class I Abs bind to the rat PMNs.

Rat PMNs were isolated, incubated with PE-labeled isotype, OX18, or OX27, and fixed in paraformaldehyde. PMNs incubated with the OX18 and OX27 antibodies bound to the PMNs, as demonstrated by the fluorescent shift compared with the labeled isotype controls via flow cytometry (n = 3).

Kelher, M. R., Masuno, T., Moore, E. E., Damle, S., Meng, X., Song, Y., ... & Gamboni-Robertson, F. (2009). Plasma from stored packed red blood cells and MHC class I antibodies causes acute lung injury in a 2-event in vivo rat model. Blood, The Journal of the American Society of Hematology, 113(9), 2079-2087.