Mouse Anti-MSH6 Recombinant Antibody (clone PT0171) (CAT#: VS3-CJ1029)

• Immunogen
• Synthesized peptide derived from human MutS protein Homolog 6 (MSH6)

• Host Species
• Mouse

• Type
• Mouse IgG1, κ

• Specificity
• Human MSH6

• Species Reactivity
• Human

• Clone
• PT0171

• Applications
• WB, IF, IHC-P

• Conjugate
• Unconjugated

• Purification
• Affinity Chromatography

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• 50% Glycerol, 0.5% BSA, PBS.

• Preservative
• 0.02% Sodium Azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• This antibody has been tested for use in Western Blot (1:500-1:2000), Immunofluorescence (1:500-1:200), Immunohistochemistry-Parraffin (1:100-1:500).

• Alternative Names
• GTBP; HSAP; p160; GTMBP; MSH-6; HNPCC5; MMRCS3

• Gene ID
• 2956

• UniProt ID
• P52701

• Sequence Similarities
• Belongs to the DNA mismatch repair MutS family.

• Cellular Localization
• Chromosome, Nucleus

• Post Translation Modifications
• The N-terminus is blocked.
  Phosphorylated by PRKCZ, which may prevent MutS alpha degradation by the ubiquitin-proteasome pathway.

• Protein Refseq
• NP_000170.1; NP_001268421.1; NP_001268422.1

• Function
• Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction.

Secondary Antibody

• 2RD-A1460-YJ
• Goat Anti-Mouse IgG1 Secondary antibody

• 2RD-A1562-YJ
• Rabbit Anti-Mouse IgG1 Secondary antibody (Clone RM106)

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS