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Recombinant Mouse Anti-MSH6 Antibody (clone 2E3A10) (CAT#: VS3-FY961)

This product is a recombinant mouse antibody that recognizes MSH6. This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Flow Cytometry. The clone 2E3A10 is specific for MSH6.


See more Engineered Antibody products for MSH6
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28376/if_selected.jpg
Figure 2 IHC staining of human lymph node
Figure 2 IHC staining of human lymph node
(Immunohistochemical staining of human lymph node shows strong nuclear positivity in reaction center cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9091/ihc_selected.jpg
Figure 3 IHC staining of human colon
Figure 3 IHC staining of human colon
(Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70870/ihc_selected.jpg
Figure 4 IF staining of human cell line A-431
Figure 4 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, golgi apparatus & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28376/290_E8_2_red_green.jpg
Figure 5 IF staining of human cell line U-251 MG
Figure 5 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28376/249_E8_1_red_green.jpg
Normal Tissue
Figure 6 Cerebral cortex
Figure 6 Cerebral cortex
(Glial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70870/151813_B_8_5.jpg
Figure 7 Colon
Figure 7 Colon
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70870/151813_A_7_3.jpg
Figure 8 Liver
Figure 8 Liver
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70870/151813_A_9_4.jpg
Figure 9 Kidney
Figure 9 Kidney
(Cells in glomeruli
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70870/151813_A_8_5.jpg
Figure 10 Testis
Figure 10 Testis
(Elongated or late spermatids
Staining:High
Intensity: Strong
Quantity:>75%
Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Pachytene spermatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Peritubular cells
Staining:High
Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining:High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining:High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70870/151813_A_6_6.jpg
Figure 11 Ovary
Figure 11 Ovary
(Ovarian stroma cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70870/151813_A_4_7.jpg
Figure 12 Lymph node
Figure 12 Lymph node
(Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70870/151813_A_9_8.jpg
RNA Expression
Figure 13 RNA cell line category: Low cell line specificity
Figure 13 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000116062-MSH6

Specifications

  • Host Species
  • Mouse
  • Type
  • Mouse IgG2b
  • Clone
  • 2E3A10
  • Applications
  • Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Flow Cytometry
  • Conjugate
  • Unconjugated

Product Property

  • Purity
  • >95% as determined by SDS-PAGE
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Flow Cytometry.

Target

  • Alternative Names
  • MutS Homolog 6; G/T Mismatch-Binding Protein; MutS-Alpha 160 KDa Subunit; MutS Protein Homolog 6; GTMBP; GTBP; P160; DNA Mismatch Repair Protein Msh6
  • Sequence Similarities
  • Belongs to the DNA mismatch repair MutS family.
  • Cellular Localization
  • Chromosome, Nucleus
  • Post Translation Modifications
  • The N-terminus is blocked.
    Phosphorylated by PRKCZ, which may prevent MutS alpha degradation by the ubiquitin-proteasome pathway.
  • Function
  • Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction.

Recommended Products

Secondary Antibody

Isotype Control

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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Recombinant Antibody

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MRO-2045-CN Rabbit Anti-MSH6 Polyclonal Antibody (MRO-2045-CN) WB, IF, IHC, FC Rabbit IgG
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VS3-CJ1030 Rabbit Anti-MSH6 Recombinant Antibody (VS3-CJ1030) WB, ICC, IF, IHC Rabbit IgG

Rabbit Monoclonal Antibody

CAT Product Name Application Type
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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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