Rabbit Anti-NKX2-1 Recombinant Antibody (clone R07-3I9) (CAT#: VS3-XY3446)
The product is a recombinant rabbit antibody that recognizes NKX2-1 (NK2 homeobox 1), which activates the transcription of GNRHR and plays a role in enhancing the circadian oscillation of its gene expression. It is capable of binding to human, mouse and rat NKX2-1. It has been used in a variety of applications, such as Western Blot, Immunohistochemistry-Paraffin, Flow cytometry, Immunocytochemistry or Immunofluorescence.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HEK 293
(Immunofluorescent staining of human cell line HEK 293 shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/74316/1569_C4_3_selected.jpg
(Immunofluorescent staining of human cell line HEK 293 shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/74316/1569_C4_3_selected.jpg
Figure 2 Thyroid gland
(Glandular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53633/117834_B_3_5.jpg
(Glandular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/53633/117834_B_3_5.jpg
Figure 3 RNA cell line category: Cell line enriched (SCLC-21H)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136352-NKX2-1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136352-NKX2-1
Figure 3 RNA cell line category: Cell line enriched (SCLC-21H)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136352-NKX2-1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136352-NKX2-1
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat NKX2-1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R07-3I9
- Applications
- WB, IHC-P, ICC, IF, FC
- Related Disease
- Chorea, Benign Hereditary; Choreoathetosis
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
FC: 1/50-1/100
Target
- Alternative Names
- BCH; BHC; NK-2; TEBP; TTF1; NKX2A; NMTC1; T/EBP; TITF1; TTF-1; NKX2.1
- Gene ID
- 7080
- UniProt ID
- P43699
- Long Name
- NK2 homeobox 1
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Phosphorylated on serine residues by STK3/MST2. (P43699-NKX21_HUMAN)
Modification sites at PhosphoSitePlus (P43699)
Modification sites at neXtProt (NX_P43699)
Glycosylation from GlyGen (P43699) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_003308.1; NP_001073136.1
- Function
- Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor.
Crucial in the maintenance of the thyroid differentiation phenotype.
May play a role in lung development and surfactant homeostasis.
Forms a regulatory loop with GRHL2 that coordinates lung epithelial cell morphogenesis and differentiation.
Activates the transcription of GNRHR and plays a role in enhancing the circadian oscillation of its gene expression.
Represses the transcription of the circadian transcriptional repressor NR1D1.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "NKX2-1"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0342MZ | Recombinant Mouse Anti-Human NK2 Homeobox 1 Antibody (clone 9H8G4/2) | IHC | Mouse antibody |
| ZG-0363U | Rabbit Anti-NKX2-1 Recombinant Antibody (clone A22-A) | ELISA, WB, IP | Rabbit IgG |
| ZG-0364U | Rabbit Anti-NKX2-1 Recombinant Antibody (clone G21-G) | IHC-P | Rabbit IgG |
| ZG-0487U | Rabbit Anti-NKX2-1 Recombinant Antibody (clone 6C11) | ELISA, WB, IHC, FC | Rabbit IgG |
| VS3-FY1045 | Recombinant Rabbit Anti-NKX2-1 Antibody (clone R02-6A5) | WB, IHC-P, IP | Rabbit IgG |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2449 | Hi-Affi™ Recombinant Rabbit Anti-NKX2-1 Monoclonal Antibody (DS2449AB) | IHC-P | IgG |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for VS3-XY3446. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.