Mouse Anti-NOTCH2 Recombinant Antibody (clone B9) (CAT#: FAMAB-1387CQ)
This product is a recombinant mouse antibody that recognizes NOTCH2. The antibody was purified by affinity chromatography. It can be used in ELISA, IHC.
Specific Inquiry
Inhib
Figure 1 Blocking Notch2 antibody inhibits the effect of MINAR1 in tube formation of endothelial cells.
Pictures were taken after overnight and quantified via Image J/angiogenesis assay.
Ho, R. X. Y., Meyer, R. D., Chandler, K. B., Ersoy, E., Park, M., Bondzie, P. A., ... & Rahimi, N. (2018). MINAR1 is a Notch2-binding protein that inhibits angiogenesis and breast cancer growth. Journal of molecular cell biology, 10(3), 195-204.
Block
Figure 2 Blocking Notch2 antibody inhibits the effect of MINAR1 in tube formation of endothelial cells.
PAE cells expressing empty vector or MINAR1 were subjected to a matrigel capillary tube formation assay treated with conditioned medium containing blocking anti-Notch2 antibody or control medium.
Ho, R. X. Y., Meyer, R. D., Chandler, K. B., Ersoy, E., Park, M., Bondzie, P. A., ... & Rahimi, N. (2018). MINAR1 is a Notch2-binding protein that inhibits angiogenesis and breast cancer growth. Journal of molecular cell biology, 10(3), 195-204.
ELISA
Figure 3 Specificity of anti-Notch1 and anti-Notch 2 blocking antibodies.
ELISA experiments were carried out with serial dilutions of antibodies on immobilised NRR domains ofmouse Notch1.
Falk, R., Falk, A., Dyson, M. R., Melidoni, A. N., Parthiban, K., Young, J. L., ... & McCafferty, J. (2012). Generation of anti-Notch antibodies and their application in blocking Notch signalling in neural stem cells. Methods, 58(1), 69-78.
IF
Figure 4 Inhibition of Notch in human NES cells promotes neuronal differentiation.
NES cells were differentiated for 7 days treated with antibody blockade of Notch1-3. Cells were then stained with the post-mitotic neuronal marker beta-III-tubulin (Tuj1) which showed that inhibition of Notch increased the amount of Tuj1 + cells.
Falk, R., Falk, A., Dyson, M. R., Melidoni, A. N., Parthiban, K., Young, J. L., ... & McCafferty, J. (2012). Generation of anti-Notch antibodies and their application in blocking Notch signalling in neural stem cells. Methods, 58(1), 69-78.
Specifications
- Immunogen
- NRR of mouse Notch 2
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Mouse mNRR2 and human hNRR2
- Species Reactivity
- Human, Mouse
- Clone
- B9
- Applications
- Inhib, BL, ELISA, IF
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Inhibition, Blocking, Immunofluorescence and Enzyme-Linked Immunosorbent Assay.
Target
- Alternative Names
- Notch 2; HN2; Neurogenic Locus Notch Homolog Protein 2; Notch (Drosophila) Homolog 2; Notch Homolog 2 (Drosophila); Notch Homolog 2; HJCYS; AGS2;
- Gene ID
- 4853
- UniProt ID
- Q04721
Related Resources
Breast Cancer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "NOTCH2"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2555z | Mouse Anti-NOTCH2 Recombinant Antibody (clone 19B5) | ELISA, IHC | Mouse IgG1, κ |
| HPAB-0595-WJ | Human Anti-NOTCH2 Recombinant Antibody (clone D) | ELISA, WB | Human IgG |
| HPAB-0596-WJ | Human Anti-NOTCH2 Recombinant Antibody (clone D-1) | ELISA, WB | Human IgG |
| HPAB-0597-WJ | Human Anti-NOTCH2 Recombinant Antibody (clone D-2) | ELISA, WB | Human IgG |
| VS3-FY1050 | Recombinant Mouse Anti-NOTCH2 Antibody (clone 2D1B2) | ELISA, IHC, ICC, FC | Mouse IgG2a |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-H67 | Anti-Human NOTCH2 Recombinant Antibody (Tarextumab) | FuncS, Block, ELISA | IgG2 - kappa |
| TAB-552MZ | Human Anti-NOTCH2 Recombinant Antibody (TAB-552MZ) | ELISA | Human IgG |
| TAB-552MZ-S(P) | Human Anti-NOTCH2 Recombinant Antibody; scFv Fragment (TAB-552MZ-S(P)) | ELISA | Human scFv |
| TAB-552MZ-F(E) | Human Anti-NOTCH2 Recombinant Antibody; Fab Fragment (TAB-552MZ-F(E)) | ELISA | Human Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0038-CN-S(P) | Human Anti-NOTCH2 Recombinant Antibody; scFv Fragment (HPAB-0038-CN-S(P)) | FC, ELISA | Human scFv |
| HPAB-0039-CN-S(P) | Human Anti-NOTCH2 Recombinant Antibody; scFv Fragment (HPAB-0039-CN-S(P)) | FC, ELISA | Human scFv |
| HPAB-0595-WJ-S(P) | Human Anti-NOTCH2 Recombinant Antibody (clone D); scFv Fragment | ELISA, WB | Human scFv |
| HPAB-0596-WJ-S(P) | Human Anti-NOTCH2 Recombinant Antibody (clone D-1); scFv Fragment | ELISA, WB | Human scFv |
| HPAB-0597-WJ-S(P) | Human Anti-NOTCH2 Recombinant Antibody (clone D-2); scFv Fragment | ELISA, WB | Human scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0038-CN-F(E) | Human Anti-NOTCH2 Recombinant Antibody; Fab Fragment (HPAB-0038-CN-F(E)) | FC, ELISA | Human Fab |
| HPAB-0039-CN-F(E) | Human Anti-NOTCH2 Recombinant Antibody; Fab Fragment (HPAB-0039-CN-F(E)) | FC, ELISA | Human Fab |
| HPAB-0595-WJ-F(E) | Human Anti-NOTCH2 Recombinant Antibody (clone D); Fab Fragment | ELISA, WB | Human Fab |
| HPAB-0596-WJ-F(E) | Human Anti-NOTCH2 Recombinant Antibody (clone D-1); Fab Fragment | ELISA, WB | Human Fab |
| HPAB-0597-WJ-F(E) | Human Anti-NOTCH2 Recombinant Antibody (clone D-2); Fab Fragment | ELISA, WB | Human Fab |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-H67 | Afuco™ Anti-NOTCH2 ADCC Recombinant Antibody (Tarextumab), ADCC Enhanced | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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