Rabbit Anti-Phospho-POLR2A (S5) Antibody (clone 2H4) displayed high specificity against the POLR2A protein of Human.
Figure 1 Rabbit Anti-Phospho-POLR2A (S5) Antibody (ZG-0515U) in WB
Western Blot
Positive WB detected in Hela whole cell lysate, A549 whole cell lysate, 293 whole cell lysate
All lanes Phospho-POLR2A antibody at 0.75μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 270 KDa
Observed band size: 270 KDa
Figure 2 Rabbit Anti-Phospho-POLR2A (S5) Antibody (ZG-0515U) in ICC
Immunocytochemistry analysis of ZG-0515U diluted at 1:75 and staining in Hela cells(treated with 100ng/ml EGF for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Figure 3 Rabbit Anti-Phospho-POLR2A (S5) Antibody (ZG-0515U) in IF
Immunofluorescence staining of Hela cells with ZG-0515U at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-2422z | Mouse Anti-POLR2A Recombinant Antibody (clone 23G8) | WB, ELISA, FC, ICC, IF, IP | Mouse IgG1 |
MRO-2362-CN | Recombinant Rabbit Anti-POLR2A (phosphorylated Ser2) Monoclonal Antibody (JM11-51) | WB, IP, IF, IHC, FC | Rabbit IgG |
MRO-2363-CN | Recombinant Rabbit Anti-POLR2A (phosphorylated Ser5) Monoclonal Antibody (JM51-21) | WB, IP, IF, IHC, FC | Rabbit IgG |
ZG-0514U | Rabbit Anti-Phospho-POLR2A (S2) Recombinant Antibody (clone 2G1) | ELISA, WB, IHC, IF, IP | Rabbit IgG |
VS3-CJ812 | Mouse Anti-POLR2A Recombinant Antibody (clone 1357CT288.1.37) | ELISA, WB | Mouse IgG1, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-2782 | Hi-Affi™ Recombinant Rabbit Anti-POLR2A Monoclonal Antibody (DS2782AB) | WB, IP, IHC-P, ICC/IF | IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MHC-LC2084 | PE-B*07:02/Human POLR2A (TPQSNRPVM) MHC Tetramer | FCM | |
MHC-LC2085 | APC-B*07:02/Human POLR2A (TPQSNRPVM) MHC Tetramer | FCM |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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