Rabbit Anti-Phospho-RAF1 (S259) Recombinant Antibody (clone 4C9) (CAT#: ZG-0540U)

Figure 1 Rabbit Anti-Phospho-RAF1 (S259) Antibody (ZG-0540U) in WB

Western Blot
Positive WB detected in Hela whole cell lysate(treated with Calyculin A or EGF)
All lanes Phospho-RAF1 antibody at 1.18μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 73 KDa
Observed band size: 73 KDa

Figure 2 Rabbit Anti-Phospho-RAF1 (S259) Antibody (ZG-0540U) in IHC

IHC image of ZG-0540U diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Figure 3 Rabbit Anti-Phospho-RAF1 (S259) Antibody (ZG-0540U) in IF

Immunofluorescence staining of HepG2 cells with ZG-0540U at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).