Rabbit Anti-Phospho-RAF1 (S43) Recombinant Antibody (clone 1F7) (CAT#: ZG-0541U)

Rabbit Anti-Phospho-RAF1 (S43) Antibody (clone 1F7) displayed high specificity against the RAF1 protein of Human.


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IF

Figure 1 Rabbit Anti-Phospho-RAF1 (S43) Antibody (ZG-0541U) in IF

Figure 1 Rabbit Anti-Phospho-RAF1 (S43) Antibody (ZG-0541U) in IF

Immunofluorescence staining of Hela with ZG-0541U at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).


Specifications

  • Immunogen
  • A synthesized peptide derived from human Phospho-RAF1 (S43)
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human Phospho-RAF1 (S43)
  • Species Reactivity
  • Human
  • Clone
  • 1F7
  • Applications
  • ELISA, IF
  • Conjugate
  • Unconjugated
  • Related Disease
  • Noonan Syndrome 5 and Leopard Syndrome 2.

Product Property

  • Purification
  • Affinity chromatography purified
  • Buffer
  • PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Preservative
  • 0.02% sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Immunofluorescence. Specific conditions should be optimized by the end user.
    IF:1:20-1:200

Target

  • Alternative Names
  • RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1; RAF1; RAF

Related Resources

  • Related Signaling Pathways
  • Related Diseases

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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