Rabbit Anti-PKN2 Recombinant Antibody (clone R02-5K8) (CAT#: VS3-XY3467)
The antibody is a recombinant rabbit antibody against human PKN2 (Protein kinase N2), which plays a role in the regulation of cell cycle progression, actin cytoskeleton assembly and cell migration. The antibody also has cross-reactivity against mouse and rat PKN2. It has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Flow cytometry, Immunocytochemistry or Immunofluorescence.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & intermediate filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34861/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & intermediate filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34861/if_selected.jpg
Figure 2 IHC staining of human kidney
(Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in glomeruli.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34861/ihc_selected.jpg
(Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in glomeruli.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/34861/ihc_selected.jpg
Figure 3 IHC staining of human breast
(Immunohistochemical staining of human breast shows moderate cytoplasmic and nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57913/164559_B_2_4_selected.jpg
(Immunohistochemical staining of human breast shows moderate cytoplasmic and nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57913/164559_B_2_4_selected.jpg
Figure 4 Cerebral cortex
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57913/164559_B_9_5.jpg
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57913/164559_B_9_5.jpg
Figure 5 Colon
(Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57913/164559_A_7_3.jpg
(Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57913/164559_A_7_3.jpg
Figure 6 Liver
(Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57913/164559_A_8_4.jpg
(Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57913/164559_A_8_4.jpg
Figure 7 Testis
(Spermatogonia cells
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57913/164559_A_4_6.jpg
(Spermatogonia cells
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57913/164559_A_4_6.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000065243-PKN2
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000065243-PKN2
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat PKN2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R02-5K8
- Applications
- WB, IHC-P, ICC, IF, IP, FC
- Related Disease
- Renal Malformations; Bubonic Plague
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
IP: 1/20
FC: 1/50-1/100
Target
- Alternative Names
- PAK2; PRK2; STK7; Pak-2; PRKCL2; PRO2042
- Gene ID
- 5586
- UniProt ID
- Q16513
- Long Name
- Protein kinase N2
- Sequence Similarities
- Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
- Cellular Localization
- Cell junction, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Nucleus
- Post Translation Modifications
- Autophosphorylated. Phosphorylated during mitosis. Phosphorylated by CDK10 (PubMed:27104747).
Activated by limited proteolysis with trypsin (By similarity).
Proteolytically cleaved by caspase-3 during the induction of apoptotic cell death.
- Protein Refseq
- NP_001307636.1; NP_001307637.1; NP_001307638.1
- Function
- PKC-related serine/threonine-protein kinase and Rho/Rac effector protein that participates in specific signal transduction responses in the cell. Plays a role in the regulation of cell cycle progression, actin cytoskeleton assembly, cell migration, cell adhesion, tumor cell invasion and transcription activation signaling processes. Phosphorylates CTTN in hyaluronan-induced astrocytes and hence decreases CTTN ability to associate with filamentous actin.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "PKN2"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0782MZ | Recombinant Mouse Anti-Human PKN2 Antibody | WB | Mouse antibody |
| VS3-QX883 | Mouse Anti-PKN2 Recombinant Antibody (clone 4C2-B2-A0) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS4-CJ77 | Recombinant Rabbit Anti-PKN2 Antibody (clone 20A8) | ELISA, WB, IHC, IF, FC | Rabbit IgG |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2727 | Hi-Affi™ Recombinant Rabbit Anti-PKN2 Monoclonal Antibody (DS2727AB) | WB, IHC-P, ICC, FC, IP | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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