Recombinant Rabbit Anti-PKN2 Antibody (clone 20A8) (CAT#: VS4-CJ77)

• Immunogen
• A synthesized peptide derived from human PKN2

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human PKN2

• Species Reactivity
• Human

• Clone
• 20A8

• Applications
• ELISA, WB, IHC, IF, FC

• Conjugate
• Unconjugated

• Purification
• Affinity chromatography

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• 50% Glycerol, 150 mM NaCl, PBS, pH 7.4.

• Preservative
• 0.02% Sodium Azide

• Storage
• Store at -20°C or -80°C upon receipt. Avoid repeated freeze.

• Application Notes
• This antibody has been tested for use in ELISA, Western Blot (1:500-1:2000), Immunohistochemistry (1:50-1:200), Immunofluorescence (1:50-1:200), Flow Cytometry (1:50-1:200).

• Alternative Names
• PAK2; PRK2; STK7; Pak-2; PRKCL2; PRO2042

• Gene ID
• 5586

• UniProt ID
• Q16513

• Long Name
• Protein kinase N2

• Sequence Similarities
• Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

• Cellular Localization
• Cell junction, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Nucleus

• Post Translation Modifications
• Autophosphorylated. Phosphorylated during mitosis. Phosphorylated by CDK10 (PubMed:27104747).
  Activated by limited proteolysis with trypsin (By similarity).
  Proteolytically cleaved by caspase-3 during the induction of apoptotic cell death.

• Protein Refseq
• NP_001307636.1; NP_001307637.1; NP_001307638.1

• Function
• PKC-related serine/threonine-protein kinase and Rho/Rac effector protein that participates in specific signal transduction responses in the cell. Plays a role in the regulation of cell cycle progression, actin cytoskeleton assembly, cell migration, cell adhesion, tumor cell invasion and transcription activation signaling processes. Phosphorylates CTTN in hyaluronan-induced astrocytes and hence decreases CTTN ability to associate with filamentous actin. Phosphorylates HDAC5, therefore lead to impair HDAC5 import. Direct RhoA target required for the regulation of the maturation of primordial junctions into apical junction formation in bronchial epithelial cells. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Stimulates FYN kinase activity that is required for establishment of skin cell-cell adhesion during keratinocytes differentiation. Regulates epithelial bladder cells speed and direction of movement during cell migration and tumor cell invasion. Inhibits Akt pro-survival-induced kinase activity. Mediates Rho protein-induced transcriptional activation via the c-fos serum response factor (SRF). Involved in the negative regulation of ciliogenesis (PubMed:27104747).

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-057CQ
• Donkey Anti-Rabbit IgG (H&L) Antibody

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS