Mouse Anti-PXN Recombinant Antibody (clone LD2 sAB) (CAT#: FAMAB-1492CQ)

Figure 1 Immunostaining of native paxillin in A549 cells with Cy-5 labeled LD2 and LD4 sABs.

A549 cells were stained with Cy-5 labeled LD2 and LD4 sAB (red) or were left untreated as a control. Arrowheads point to sites where sABs are bound to paxillin present in focal adhesions. Nuclei were counterstained with Hoehst (blue) and actin filaments were stained with Alexa-488 phalloidin (green). Scale bar corresponds to 10μm. Top panel: isolated red channel representing fluorescence signal from Cy-5 labeled sAB only. Bottom panel: three merged channels representing fluorescence signals from nuclear, actin and paxillin staining.

Nocula-Lugowska, M., Lugowski, M., Salgia, R., & Kossiakoff, A. A. (2015). Engineering synthetic antibody inhibitors specific for LD2 or LD4 motifs of paxillin. Journal of molecular biology, 427(15), 2532-2547.

Figure 2 The sABs pull-down paxillin-FAK complexes.

Paxillin was immunoprecipiatated from A549 lung cancer cell lysate with LD2 and LD4 sABs and the pulled down complexes were visualized either by anti-paxillin or anti-FAK antibody.

Nocula-Lugowska, M., Lugowski, M., Salgia, R., & Kossiakoff, A. A. (2015). Engineering synthetic antibody inhibitors specific for LD2 or LD4 motifs of paxillin. Journal of molecular biology, 427(15), 2532-2547.