Rabbit Anti-PXN Recombinant Antibody (VS3-CJ984) (CAT#: VS3-CJ984)
This product is a rabbit antibody that recognizes human, mouse, and rat PXN.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & focal adhesion sites)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51309/795_G6_5_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & focal adhesion sites)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51309/795_G6_5_selected.jpg
Figure 2 IHC staining of human cerebellum
(Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3841/ihc_selected.jpg
(Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3841/ihc_selected.jpg
Figure 3 Colon
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3841/10426_A_9_3.jpg
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3841/10426_A_9_3.jpg
Figure 4 Cerebral cortex
(Endothelial cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3841/10426_B_7_5.jpg
(Endothelial cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3841/10426_B_7_5.jpg
Figure 5 Testis
(Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Pachytene spermatocytes
Staining: Low
Intensity: Moderate
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3841/10426_A_4_6.jpg
(Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Pachytene spermatocytes
Staining: Low
Intensity: Moderate
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3841/10426_A_4_6.jpg
Figure 6 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000089159-PXN/subcellular
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000089159-PXN/subcellular
Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat PXN
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IHC, IP
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation.
Target
- Alternative Names
- Paxillin
- Gene ID
- 5829
- UniProt ID
- P49023
- Sequence Similarities
- Belongs to the paxillin family.
- Cellular Localization
- Cell junction, Cytoplasm, Cytoskeleton
- Post Translation Modifications
- Phosphorylated by MAPK1/ERK2 (By similarity).
Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. Phosphorylation at Ser-244 by CDK5 reduces its interaction with PTK2/FAK1 in matrix-cell focal adhesions (MCFA) during oligodendrocytes (OLs) differentiation. Phosphorylation at Tyr-31 and Tyr-118 by PTK6 promote the activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion. Phosphorylation at Ser-250 by SLK is required for PXN redistribution and cell motility (PubMed:23128389).
- Protein Refseq
- NP_001074324.1; NP_001230685.1; NP_002850.2
- Function
- Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "PXN"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-314 | Human Anti-PXN Recombinant Antibody (clone CBL14Q) | WB, ELISA, IF, FuncS | Human IgG |
| PABL-674 | Human Anti-PXN Recombinant Antibody (clone CBL19Q) | WB, FuncS | Human IgG |
| PABL-675 | Human Anti-PXN Recombinant Antibody (clone CBL35Q) | WB, FuncS | Human IgG |
| MOB-1558CT | Recombinant Mouse anti-Human PXN Monoclonal antibody (N203) | ELISA, WB | |
| FAMAB-1491CQ | Mouse Anti-PXN Recombinant Antibody (clone LD4 sAB) | WB, ELISA | Mouse IgG1 |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-314 | Human Anti-PXN Recombinant Antibody (clone CBL14Q); scFv Fragment | WB, ELISA, IF, FuncS | Human scFv |
| PSBL-668 | Human Anti-PXN Recombinant Antibody (clone CBL19Q); scFv Fragment | WB, FuncS | Human scFv |
| PSBL-669 | Human Anti-PXN Recombinant Antibody (clone CBL35Q); scFv Fragment | WB, FuncS | Human scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-314 | Human Anti-PXN Recombinant Antibody (clone CBL14Q); Fab Fragment | WB, ELISA, IF, FuncS | Human Fab |
| PFBL-668 | Human Anti-PXN Recombinant Antibody (clone CBL19Q); Fab Fragment | WB, FuncS | Human Fab |
| PFBL-669 | Human Anti-PXN Recombinant Antibody (clone CBL35Q); Fab Fragment | WB, FuncS | Human Fab |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2947 | Hi-Affi™ Recombinant Rabbit Anti-PXN Monoclonal Antibody (DS2947AB) | FC, ICC, IHC-P, IP, WB | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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