Mouse Anti-PXN Recombinant Antibody (clone LD4 sAB) (CAT#: FAMAB-1491CQ)

This product is a recombinant mouse antibody that recognizes PXN. The antibody was purified by affinity chromatography. It can be used in WB, ELISA.


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IP

Figure 1 The sABs recognize full-length native paxillin in cellular environment.

Figure 1 The sABs recognize full-length native paxillin in cellular environment.

Top panel: immunoprecipitation of endogenous paxillin from cell lysates using LD2 and LD4 sABs. Native paxillin from lung cancer A549 cell line lysates was immunoprecipitated with increasing amounts of anti LD2 sAB and anti LD4 sABs resulting in detection of increasing amounts of paxillin of proper 68 kDa mass by anti-human paxillin IgG on Western Blot. Bottom panel: schematic representation of sABs bound to native paxillin molecule.

Nocula-Lugowska, M., Lugowski, M., Salgia, R., & Kossiakoff, A. A. (2015). Engineering synthetic antibody inhibitors specific for LD2 or LD4 motifs of paxillin. Journal of molecular biology, 427(15), 2532-2547.


Specifications

  • Immunogen
  • Peptide (WGGSATRELDELMASLSD) corresponding to human paxillin-LD4 motif (aa 260-278) fused to MBP
  • Host Species
  • Mouse
  • Type
  • Mouse IgG1
  • Specificity
  • LD-4 motif of human paxillin
  • Species Reactivity
  • Human
  • Clone
  • LD4 sAB
  • Applications
  • WB, ELISA

Product Property

  • Purity
  • >95% as determined by SDS-PAGE
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • Paxillin; PXN

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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