Rabbit Anti-RAB5A Recombinant Antibody (VS3-CJ771) (CAT#: VS3-CJ771)

• Immunogen
• Recombinant protein

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse, Rat RAB5A

• Species Reactivity
• Human, Mouse, Rat

• Applications
• WB, IHC

• Conjugate
• Unconjugated

• Purification
• Protein A affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• 40% Glycerol, 1% BSA, TBS, pH7.4.

• Preservative
• 0.05% Sodium Azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• This antibody has been tested for use in Western Blot, Immunohistochemistry.

• Alternative Names
• RAB5

• Gene ID
• 5868

• UniProt ID
• P20339

• Sequence Similarities
• Belongs to the small GTPase superfamily. Rab family.

• Cellular Localization
• Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Endosome, Membrane

• Post Translation Modifications
• Phosphorylation of Ser-84 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including RAB GDP dissociation inhibitors GDI1 and GDI2.
  (Microbial infection) Glycosylated on arginine residues by S.typhimurium protein Ssek3.

• Protein Refseq
• NP_001278977.1; NP_004153.2

• Function
• Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Active GTP-bound form is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes (PubMed:10818110, PubMed:14617813, PubMed:16410077, PubMed:15378032).
  Contributes to the regulation of filopodia extension (PubMed:14978216).
  Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (PubMed:22660413).
  Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 (By similarity).

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-057CQ
• Donkey Anti-Rabbit IgG (H&L) Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody