Recombinant Rabbit Anti-RAB5A Antibody (clone R06-4J3) (CAT#: VS3-FY1233)

• Immunogen
• Synthetic peptide of human Rab5.

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse, Rat RAB5A

• Species Reactivity
• Human, Mouse, Rat

• Clone
• R06-4J3

• Applications
• Western Blot, Immunohistochemistry-Paraffin

• Conjugate
• Unconjugated

• MW
• Calculated MW: 24 kDa; Observed MW: 24 kDa

• Purification
• Affinity Purified

• Purity
• >95% as determined by SDS-PAGE

• Buffer
• 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA

• Preservative
• 0.01% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunohistochemistry-Paraffin: 1/50-1/100

• Alternative Names
• RAB5A, Member RAS Oncogene Family; RAS-Associated Protein RAB5A; RAB5; Ras-Related Protein Rab-5A

• Gene ID
• 5868

• UniProt ID
• P20339

• Sequence Similarities
• Belongs to the small GTPase superfamily. Rab family.

• Cellular Localization
• Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Endosome, Membrane

• Post Translation Modifications
• Phosphorylation of Ser-84 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including RAB GDP dissociation inhibitors GDI1 and GDI2.
  (Microbial infection) Glycosylated on arginine residues by S.typhimurium protein Ssek3.

• Protein Refseq
• NP_001278977.1; NP_004153.2

• Function
• Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Active GTP-bound form is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes (PubMed:10818110, PubMed:14617813, PubMed:16410077, PubMed:15378032).
  Contributes to the regulation of filopodia extension (PubMed:14978216).
  Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (PubMed:22660413).
  Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 (By similarity).

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody

• 2ND-057CQ
• Donkey Anti-Rabbit IgG (H&L) Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-060CQ
• Llama Anti-Rabbit IgG (H&L) Antibody

• 2ND-061CQ
• Sheep Anti-Rabbit IgG (H&L) Antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody