Rabbit Anti-RPS3 Recombinant Antibody (VS3-CJ799) (CAT#: VS3-CJ799)

• Immunogen
• Recombinant protein

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse, Rat RPS3

• Species Reactivity
• Human, Mouse, Rat

• Applications
• WB, ICC, IHC

• Conjugate
• Unconjugated

• Purification
• Protein A affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• 40% Glycerol, 1% BSA, TBS, pH7.4.

• Preservative
• 0.05% Sodium Azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunohistochemistry.

• Alternative Names
• S3

• Gene ID
• 6188

• UniProt ID
• P23396

• Sequence Similarities
• Belongs to the universal ribosomal protein uS3 family.

• Cellular Localization
• Cytoplasm, Cytoskeleton, Membrane, Mitochondrion, Mitochondrion inner membrane, Nucleus

• Post Translation Modifications
• Methylation by PRMT1 is required for import into the nucleolus and for ribosome assembly.
  Sumoylation by SUMO1 enhances protein stability through increased resistance to proteolysis. Sumoylation occurs at one or more of the three consensus sites, Lys-18, Lys-214 and Lys-230.
  Phosphorylation at Thr-221 by CDK1 ^oCcurs mainly in G2/M phase (PubMed:21871177).
  Phosphorylation by PRKCD occurs on a non-ribosomal-associated form which results in translocation of RPS3 to the nucleus and enhances its endonuclease activity (PubMed:19059439).
  Phosphorylated on Ser-209 by IKKB in response to activation of the NF-kappa-B p65-p50 complex which enhances the association of RPS3 with importin-alpha and mediates the nuclear translocation of RPS3 (PubMed:21399639).
  Phosphorylation by MAPK is required for translocation to the nucleus following exposure of cells to DNA damaging agents such as hydrogen peroxide (PubMed:17560175).
  Phosphorylation by PKB/AKT mediates RPS3 nuclear translocation, enhances RPS3 endonuclease activity and suppresses RPS3-induced neuronal apoptosis (PubMed:20605787).
  Ubiquitinated (PubMed:16314389).
  This is prevented by interaction with HSP90 which stabilizes the protein (PubMed:16314389).
  Monoubiquitinated at Lys-214 by ZNF598 when a ribosome has stalled during translation of poly(A) sequences, leading to preclude synthesis of a long poly-lysine tail and initiate the ribosome quality control (RQC) pathway to degrade the potentially detrimental aberrant nascent polypeptide (PubMed:28065601, PubMed:28132843).
  Ufmylated by UFL1.

• Protein Refseq
• NP_000996.2; NP_001243731.1; NP_001247435.1

• Function
• Involved in translation as a component of the 40S small ribosomal subunit (PubMed:8706699).
  Has endonuclease activity and plays a role in repair of damaged DNA (PubMed:7775413).
  Cleaves phosphodiester bonds of DNAs containing altered bases with broad specificity and cleaves supercoiled DNA more efficiently than relaxed DNA (PubMed:15707971).
  Displays high binding affinity for 7,8-dihydro-8-oxoguanine (8-oxoG), a common DNA lesion caused by reactive oxygen species (ROS) (PubMed:14706345).
  Has also been shown to bind with similar affinity to intact and damaged DNA (PubMed:18610840).
  Stimulates the N-glycosylase activity of the base excision protein OGG1 (PubMed:15518571).
  Enhances the uracil excision activity of UNG1 (PubMed:18973764).
  Also stimulates the cleavage of the phosphodiester backbone by APEX1 (PubMed:18973764).
  When located in the mitochondrion, reduces cellular ROS levels and mitochondrial DNA damage (PubMed:23911537).
  Has also been shown to negatively regulate DNA repair in cells exposed to hydrogen peroxide (PubMed:17049931).
  Plays a role in regulating transcription as part of the NF-kappa-B p65-p50 complex where it binds to the RELA/p65 subunit, enhances binding of the complex to DNA and promotes transcription of target genes (PubMed:18045535).
  Represses its own translation by binding to its cognate mRNA (PubMed:20217897).
  Binds to and protects TP53/p53 from MDM2-mediated ubiquitination (PubMed:19656744).
  Involved in spindle formation and chromosome movement during mitosis by regulating microtubule polymerization (PubMed:23131551).
  Involved in induction of apoptosis through its role in activation of CASP8 (PubMed:14988002).
  Induces neuronal apoptosis by interacting with the E2F1 transcription factor and acting synergistically with it to up-regulate pro-apoptotic proteins BCL2L11/BIM and HRK/Dp5 (PubMed:20605787).
  Interacts with TRADD following exposure to UV radiation and induces apoptosis by caspase-dependent JNK activation (PubMed:22510408).

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-057CQ
• Donkey Anti-Rabbit IgG (H&L) Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody