F26G19 is a neutralizing mouse monoclonal antibody, elicited by immunization with chemically inactivated SARS-CoV. The RBD-F26G19 Fab complex represents the first example of the structural characterization of an antibody elicited by an immune response to SARS-CoV or any fragment of it. The structure reveals that the RBD surface recognized by F26G19 overlaps significantly with the surface recognized by ACE2 and, as such, suggests that F26G19 likely neutralizes SARS-CoV by blocking the virus-host cell interaction.
Figure 1 ELISA Epitope characterisation of murine F26 series mAbs.
Among these only the neutralizing mAbs (F26G9, F26G10, F26G18, F26G19) bind a fragment (rACE2BDS) spanning the receptor binding domain (RBD) from amino acids (aa) 318-510, compared to non-neutralizing spike mAbs F26G8 which does not bind. The neutralizing mAbs F26G9, F2610, F2618 and F2619 all recognize mammalian expressed RBD 318-510 fragment in ELISA.
Berry, J. D., Hay, K., Rini, J. M., Yu, M., Wang, L., Plummer, F. A., ... & Andonov, A. (2010, January). Neutralizing epitopes of the SARS-CoV S-protein cluster independent of repertoire, antigen structure or mAb technology. In MAbs (Vol. 2, No. 1, pp. 53-66). Taylor & Francis.
Figure 2 Competition ELISA with biotinylated mAbs to whole inactivated SARs-CoV.
There is a non-neutralizing epitope bin where F26G8 and competes with biot-F26G6 to greater than a 90% reduction in binding. Next is the biot-F26G9 neutralizing mAb bin which overlaps with all neutralizing mAbs (F26G7, F26G10, F26G18, F26G19). The biot-F26G18 bin overlaps by about 90% with F26G9 bin, although there is less than 50% overlap with F26G19.
Berry, J. D., Hay, K., Rini, J. M., Yu, M., Wang, L., Plummer, F. A., ... & Andonov, A. (2010, January). Neutralizing epitopes of the SARS-CoV S-protein cluster independent of repertoire, antigen structure or mAb technology. In MAbs (Vol. 2, No. 1, pp. 53-66). Taylor & Francis.
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-885-S(P) | Recombinant Anti-SARS-CoV Antibody scFv Fragment | RIA, ELISA, FuncS | scFv |
MHH-885-S(P) | Recombinant Human Anti-SARS-CoV Antibody scFv Fragment | Neut, IF, FuncS | scFv |
HPAB-S0121-YC-S(P) | Mouse Anti-SARS-CoV Recombinant Antibody (clone F26G19); scFv Fragment | Neut | Mouse scFv |
HPAB-2048-FY-S(P) | Human Anti-SARS-CoV Recombinant Antibody (clone CR03-014); scFv Fragment | Neut, ELISA, FC | Human scFv |
HPAB-2049-FY-S(P) | Human Anti-SARS-CoV Recombinant Antibody (clone CR03-022); scFv Fragment | Neut, ELISA, FC | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
MHH-885 | Recombinant Human Anti-SARS-CoV Antibody | WB, FC, FuncS | IgG |
HPAB-1909-FY | Human Anti-SARS-CoV Recombinant Antibody (clone 03-001) | ELISA | Human IgG |
HPAB-1910-FY | Human Anti-SARS-CoV Recombinant Antibody (clone 03-002) | ELISA | Human IgG |
HPAB-1911-FY | Human Anti-SARS-CoV Recombinant Antibody (clone 03-009) | ELISA, IF, EM | Human IgG |
HPAB-1912-FY | Human Anti-SARS-CoV Recombinant Antibody (clone 03-018) | ELISA, IF, EM, Inhib | Human IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MHH-885-F(E) | Recombinant Human Anti-SARS-CoV Antibody Fab Fragment | IP, RIA, FuncS | Fab |
HPAB-1909-FY-S(P) | Human Anti-SARS-CoV Recombinant Antibody (clone 03-001); Fab Fragment | ELISA | Human Fab |
HPAB-1910-FY-S(P) | Human Anti-SARS-CoV Recombinant Antibody (clone 03-002); Fab Fragment | ELISA | Human Fab |
HPAB-1911-FY-S(P) | Human Anti-SARS-CoV Recombinant Antibody (clone 03-009); Fab Fragment | ELISA | Human Fab |
HPAB-1912-FY-S(P) | Human Anti-SARS-CoV Recombinant Antibody (clone 03-018); Fab Fragment | ELISA | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-649LC | Mouse Anti-SARS-CoV Recombinant Antibody (MRO-649LC) | ELISA | Mouse antibody |
MRO-650LC | Mouse Anti-SARS-CoV Recombinant Antibody (MRO-650LC) | ELISA | Mouse antibody |
MRO-651LC | Mouse Anti-SARS-CoV Recombinant Antibody (MRO-651LC) | ELISA | Mouse antibody |
MRO-652LC | Mouse Anti-SARS-CoV Recombinant Antibody (MRO-652LC) | ELISA | Mouse antibody |
MRO-649LC-S(P) | Mouse Anti-SARS-CoV Recombinant Antibody; scFv Fragment (MRO-649LC-S(P)) | ELISA | Mouse scFv |
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