Mouse Anti-SERPINA1 Recombinant Antibody (VS3-CJ102) (CAT#: VS3-CJ102)

• Immunogen
• Recombinant fragment of human SERPINA1 (C-terminus)

• Host Species
• Mouse

• Type
• Mouse IgG

• Specificity
• Human SERPINA1

• Species Reactivity
• Human

• Applications
• WB, ELISA

• Conjugate
• Unconjugated

• Purification
• Protein G affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Concentration
• 1 mg/mL (lot specific)

• Buffer
• 50% Glycerol, 1% BSA, PBS, pH7.4.

• Preservative
• 0.02% sodium azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• This antibody has been tested for use in ELISA (1:5000-1:20000), Western Blot (1:100-1:2000).

• Alternative Names
• A1A; A1AT; AAT; PI; PI1; PRO2275; alpha1AT; Nnif

• Gene ID
• 5265

• UniProt ID
• P01009

• Sequence Similarities
• Belongs to the serpin family.

• Cellular Localization
• Endoplasmic reticulum, Extracellular matrix, Secreted

• Post Translation Modifications
• N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
  Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418.
  (Microbial infection) Proteolytically processed by Staphylococcus aureus seryl, cysteinyl, and metallo-proteases.

• Protein Refseq
• NP_000286.3; NP_001002235.1; NP_001002236.1

• Function
• Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
  Short peptide from AAT:
  Reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).

Isotype Control

• MRO-A1591-YJ
• Mouse IgG Isotype control, Polyclonal

Secondary Antibody

• 2ND-040CQ
• Goat Anti-Mouse IgG (H&L) Antibody

• 2ND-042CQ
• Rabbit Anti-Mouse IgG (H&L) Antibody

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS