Rabbit Anti-SERPINE1 Recombinant Antibody (clone 10C3) (CAT#: ZG-0077U)

The purified Rabbit Anti-SERPINE1 Recombinant Antibody (clone 10C3) displayed high specificity against the SERPINE1 protein of human.


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  • Tested Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
WB

Figure 1 Rabbit Anti-SERPINE1 Antibody (ZG-0077U) in WB

Figure 1 Rabbit Anti-SERPINE1 Antibody (ZG-0077U) in WB

Positive WB detected in: U-251 whole cell lysate, U-87 whole cell lysate. All lanes: PAI1 Antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 46, 44 kDa
Observed band size: 45 kDa

IHC

Figure 2 Rabbit Anti-SERPINE1 Antibody (ZG-0077U) in IHC

Figure 2 Rabbit Anti-SERPINE1 Antibody (ZG-0077U) in IHC

IHC image of ZG-0077U diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.


Specifications

  • Immunogen
  • A synthesized peptide derived from human PAI1
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human SERPINE1
  • Species Reactivity
  • Human
  • Clone
  • 10C3
  • Applications
  • ELISA, WB, IHC
  • Conjugate
  • Unconjugated
  • Related Disease
  • Plasminogen Activator Inhibitor-1 Deficiency and Complete Plasminogen Activator Inhibitor 1 Deficiency

Product Property

  • Purification
  • Affinity purified
  • Concentration
  • See the COA
  • Buffer
  • PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Preservative
  • 0.02% sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, western blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
    WB:1:500-1:5000, IHC:1:50-1:200

Target

  • Alternative Names
  • Plasminogen activator inhibitor 1; PAI; PAI-1; Endothelial plasminogen activator inhibitor; Serpin E1; SERPINE1; PAI1; PLANH1

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "SERPINE1"

Humanized Antibody

CAT Product Name Application Type
TAB-0925CL Human Anti-SERPINE1 Recombinant Antibody (TAB-0925CL) ELISA Humanized IgG
TAB-0926CL Anti-Human SERPINE1 Recombinant Antibody (CT110) Immunohistochemical staining, WB, ELISA Humanized antibody
TAB-0927CL Anti-Human SERPINE1 Recombinant Antibody (CT140) Immunohistochemical staining, WB, ELISA Humanized antibody
TAB-0928CL Anti-Human SERPINE1 Recombinant Antibody (55F4C12) Immunohistochemical staining, WB, ELISA Humanized antibody
TAB-0925CL-S(P) Human Anti-SERPINE1 Recombinant Antibody; scFv Fragment (TAB-0925CL-S(P)) ELISA Humanized scFv

Single-domain Antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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