Rabbit Anti-SIGLEC1 Recombinant Antibody (clone SER-4) (CAT#: FAMAB-1630CQ)

Figure 1 Inhibition of SER by mAb SER-4.

Phase contrast micrographs of rosetting assays with mouse serum-induced TPM in the absence (A) or presence (B) of SER-4 mAb tissue culture supernatant. TPM were cultured on glass coverslips for 3 d in 15% mouse serum to induce SER.They were then incubated for 1 h in the presence or absence of SER-4 mAb tissue culture supernatant. After extensive washing, sheep E were added at 0.5% vol/vol. After 30 min at 37 °C unbound sheep E were removed and the preparations were fixed in 1% glutaraldehyde before examination. (A) The majority of TPM rosette intensely with sheep E. (B) Erythrocyte binding is completely inhibited after preincubation with SER-4 mAb tissue culture supernatant.

Crocker, P. R., & Gordon, S. I. A. M. O. N. (1989). Mouse macrophage hemagglutinin (sheep erythrocyte receptor) with specificity for sialylated glycoconjugates characterized by a monoclonal antibody. The Journal of experimental medicine, 169(4), 1333-1346.

Figure 2 Inhibition of SER by mAb SER-4 IgG, F(ab')2,and Fab fragments.

TPM were induced to express SER by culture in 15% mouse serum for3 d on microtiter plates. Wells were incubated for 1 h with three fold serial dilutions of IgG, F(ab')2, or Fab, washed extensively, and assayed for sheep E binding. The data show mean values of duplicate wells. Similar results were obtained in three independent experiments.

Crocker, P. R., & Gordon, S. I. A. M. O. N. (1989). Mouse macrophage hemagglutinin (sheep erythrocyte receptor) with specificity for sialylated glycoconjugates characterized by a monoclonal antibody. The Journal of experimental medicine, 169(4), 1333-1346.

Figure 3 Western blots of SER4 Ag expression in nonreduced lysates of mouseserum-induced TPM, spleen, lymph nodes,thymus, and bone marrow.

Blots were prepared from 6.5 17o gels and probed with 1251-SER4IgG in the absence or presence (left) panel of100-fold excess unlabeled SER4 IgG. A specific band at ti 170 x 103 Mr can be seen in lysates of mouse serum-induced TPM, spleen, lymphnode, and bone marrow, but not thymus. The weak signal observed in bone marrow lysatesreflects the fact that lysates were normalized for organ input, rather than cell number. The positions of molecular weight standards(x 10 -3 ) are indicated.

Crocker, P. R., & Gordon, S. I. A. M. O. N. (1989). Mouse macrophage hemagglutinin (sheep erythrocyte receptor) with specificity for sialylated glycoconjugates characterized by a monoclonal antibody. The Journal of experimental medicine, 169(4), 1333-1346.