This product is a recombinant antibody that recognizes OPN. The monoclonal antibody 1A12 reacts with the epitope of OPN.
Figure 1 In vivo study of anti-OPN antibodies.
Lung metastasis of breast cancer xenograft model. Female nude mice were given injections of 5 × 10 6 MDA-MB-435s cells into the MFP. Representative lung tissue sections of hematoxylin and eosin staining from the nude mice after MFP injection of MDA-MB-435s cells with or without anti-OPN antibody treatment. Arrows indicate tumor metastasis sites. Anti-OPN antibodies treatment inhibited lung metastasis in the orthotopic model.
Peng, L., Guo, Y., Zhou, Y., Dai, J., & Wang, H. (2009). Inhibition of Breast Cancer Metastasis and Angiogenesis by Antiosteopontin Single-Chain Fv-Fc Fusion Protein. Neoplasia, 11(5), 509-519.
Figure 2 Antiangiogenesis effect of anti-OPN antibodies.
In some experiments, 1 μg of 1A12 and 2H8 was added simultaneously with OPN to examine its ability to inhibit the OPN-induced angiogenic response. Neovascularization was observed in OPN and VEGF group, whereas no vascular response was observed in control CAM with PBS.
Peng, L., Guo, Y., Zhou, Y., Dai, J., & Wang, H. (2009). Inhibition of Breast Cancer Metastasis and Angiogenesis by Antiosteopontin Single-Chain Fv-Fc Fusion Protein. Neoplasia, 11(5), 509-519.
Figure 3 The construction and biopanning of phage antibody library.
Competitive ELISA was performed to determine the competitive binding inhibition between phage scFv and scFv-Fc. Inhibition of phage scFv binding to OPN was observed by using scFv-Fc fusion protein as a competitor. The bound phages were detected by ELISA with HRPconjugated anti-M13 antibody.
Peng, L., Guo, Y., Zhou, Y., Dai, J., & Wang, H. (2009). Inhibition of Breast Cancer Metastasis and Angiogenesis by Antiosteopontin Single-Chain Fv-Fc Fusion Protein. Neoplasia, 11(5), 509-519.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-0846CL-S(P) | Anti-Human SPP1 Recombinant Antibody scFv Fragment (MOR-6990) | ELISA | Human antibody |
TAB-0847CL-S(P) | Anti-Human SPP1 Recombinant Antibody scFv Fragment (MOR-6991) | ELISA | Human antibody |
TAB-0848CL-S(P) | Anti-Human SPP1 Recombinant Antibody scFv Fragment (MOR-6993) | ELISA | Human antibody |
TAB-0846CL-F(E) | Anti-Human SPP1 Recombinant Antibody Fab Fragment (MOR-6990) | ELISA | Human antibody |
TAB-0847CL-F(E) | Anti-Human SPP1 Recombinant Antibody Fab Fragment (MOR-6991) | ELISA | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
BRD-0814MZ | Chicken Anti-Osteopontin Polyclonal IgY | ELISA, IHC, WB | Chicken antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-3394 | Hi-Affi™ Recombinant Rabbit Anti-SPP1 Monoclonal Antibody (DS3394AB) | FC, ICC/IF, IHC-P, WB | IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0068-YC | Human Anti-SPP1 Recombinant Antibody (clone h2K1) | FuncS | Humanized IgG |
HPAB-0569-YJ | Human Anti-SPP1 Recombinant Antibody (clone OPN-3) | ELISA | Human IgG |
ZG-0075U | Rabbit Anti-SPP1 Recombinant Antibody (clone 10A1) | ELISA, WB, IHC | Rabbit IgG |
ZG-0182U | Mouse Anti-SPP1 Recombinant Antibody (clone 1B2C2) | ELISA, WB, IHC | Mouse IgG2a |
VS3-XY1459 | Mouse Anti-SPP1 Recombinant Antibody (clone 7C5H12) | ELISA, WB, IHC, ICC | Mouse IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0068-YC-S(P) | Human Anti-SPP1 Recombinant Antibody (clone h2K1); scFv Fragment | FuncS | Humanized scFv |
HPAB-0069-YC-S(P) | Human Anti-SPP1 Recombinant Antibody (clone c2K1); scFv Fragment | FuncS | Mouse scFv |
HPAB-0070-YC-S(P) | Human Anti-SPP1 Recombinant Antibody (clone R2K1v1.7); scFv Fragment | ELISA | Humanized scFv |
HPAB-1363-FY-F(E) | Mouse Anti-SPP1 Recombinant Antibody (clone 1A12); scFv Fragment | WB, ELISA | Mouse scFv |
HPAB-1079WJ-S(P) | Human Anti-SPP1 Recombinant Antibody (clone MOR-6990); scFv Fragment | ELISA | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0068-YC-F(E) | Human Anti-SPP1 Recombinant Antibody (clone h2K1); Fab Fragment | FuncS | Humanized Fab |
HPAB-1079WJ-F(E) | Human Anti-SPP1 Recombinant Antibody (clone MOR-6990); Fab Fragment | ELISA | Human Fab |
HPAB-1080WJ-F(E) | Human Anti-SPP1 Recombinant Antibody (clone MOR-6991); Fab Fragment | ELISA | Human Fab |
HPAB-1081WJ-F(E) | Human Anti-SPP1 Recombinant Antibody (clone MOR-6993); Fab Fragment | ELISA | Human Fab |
HPAB-0569-YJ-F(E) | Human Anti-SPP1 Recombinant Antibody (clone OPN-3); Fab Fragment | ELISA | Human Fab |
There are currently no Customer reviews or questions for HPAB-1363-FY. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.