This recombinant monoclonal antibody to Human STEAP1 is a Mouse monoclonal antibody that can be used for applications: ELISA, Inhib.
Figure 1 Cell-surface ferric-reductase activity of STEAP4 and STEAP4/1 chimera while varying the concentration of added Fab120.545.
The STEAP4/1 chimera datapoints are fitted with a one-phase decay curve.
Oosterheert, W., & Gros, P. (2020). Structural basis for targeting human cancer antigen STEAP1 with antibodies. bioRxiv.
Figure 2 Cell-surface ferric-reductase activity of STEAP protein variants supplemented with PBS or with 4 µM Fab120.545.
Oosterheert, W., & Gros, P. (2020). Structural basis for targeting human cancer antigen STEAP1 with antibodies. bioRxiv.
Figure 3 PC3-STEAP-1 cells were transfected with a control siRNA (gray histogram) or a STEAP-1 siRNA (black histogram).
Cells were harvested 72 h later, stained with either isotype control mAb (solid histogram) or mAb 120.545 (open histograms), and then analyzed by FACS.
Challita-Eid, P. M., Morrison, K., Etessami, S., An, Z., Morrison, K. J., Perez-Villar, J. J., ... & Jakobovits, A. (2007). Monoclonal antibodies to six-transmembrane epithelial antigen of the prostate-1 inhibit intercellular communication in vitro and growth of human tumor xenografts in vivo. Cancer research, 67(12), 5798-5805.
Figure 4 Flow cytometry
3T3-neo, 3T3-STEAP-1, and LNCaP cells were stained with either mAb 92.30 or mAb 120.545 for 1 h at 4°C followed by phycoerythrin-conjugated antimouse IgG antibody. The cells were then analyzed by flow cytometry.
Challita-Eid, P. M., Morrison, K., Etessami, S., An, Z., Morrison, K. J., Perez-Villar, J. J., ... & Jakobovits, A. (2007). Monoclonal antibodies to six-transmembrane epithelial antigen of the prostate-1 inhibit intercellular communication in vitro and growth of human tumor xenografts in vivo. Cancer research, 67(12), 5798-5805.
Figure 5 Immunoprecipitates were prepared from cell lysates of 3T3-neo or 3T3-STEAP-1 cells with either mAb 92.30 (left) or mAb 120.545 (right).
The immunoprecipitates were subjected to SDS-PAGE, blotted, and stained with sheep STEAP-1 polyclonal antibody.
Challita-Eid, P. M., Morrison, K., Etessami, S., An, Z., Morrison, K. J., Perez-Villar, J. J., ... & Jakobovits, A. (2007). Monoclonal antibodies to six-transmembrane epithelial antigen of the prostate-1 inhibit intercellular communication in vitro and growth of human tumor xenografts in vivo. Cancer research, 67(12), 5798-5805.
Figure 6 LAPC-9AD cells were injected s.c. into SCID mice.
Mice (n = 10) were treated with either PBS, control mouse mAb, or mAbs 92.30 and 120.545 at 500 μg/dose. Treatment was given by i.p. injection, twice weekly for 6 wks starting on the day of tumor injection. Tumor volume was measured at the indicated time points. Points, mean tumor volume (mm3); bars, SE. Statistical analysis using the Kruskal-Wallis test shows P < 0.05 for both mAb 120.545 and mAb 92.30 treatments compared with PBS and control mAb treatments.
Challita-Eid, P. M., Morrison, K., Etessami, S., An, Z., Morrison, K. J., Perez-Villar, J. J., ... & Jakobovits, A. (2007). Monoclonal antibodies to six-transmembrane epithelial antigen of the prostate-1 inhibit intercellular communication in vitro and growth of human tumor xenografts in vivo. Cancer research, 67(12), 5798-5805.
Figure 7 UM-UC-3 cells were injected s.c. into SCID mice.
Treatment was given by i.p. injection with 1 mg/dose twice weekly for a total of three doses starting on day 8 (arrow). Tumor volume was measured at the indicated time points. Points, mean tumor volume (mm3); bars, SE. Statistical analysis using the Dunnett test shows P < 0.01 for both mAb 120.545 and mAb 92.30 treatments compared with control treatment.
Challita-Eid, P. M., Morrison, K., Etessami, S., An, Z., Morrison, K. J., Perez-Villar, J. J., ... & Jakobovits, A. (2007). Monoclonal antibodies to six-transmembrane epithelial antigen of the prostate-1 inhibit intercellular communication in vitro and growth of human tumor xenografts in vivo. Cancer research, 67(12), 5798-5805.
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• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1961CT | Recombinant Mouse anti-Human STEAP1 Monoclonal antibody (EML1413) | WB | |
HPAB-N0259-YC | Human Anti-STEAP1 Recombinant Antibody (HPAB-N0259-YC) | FuncS | Humanized IgG |
HPAB-0487-YJ | Mouse Anti-STEAP1 Recombinant Antibody (HPAB-0487-YJ) | FC, ELISA | Mouse IgG1 |
HPAB-0488-YJ | Human Anti-STEAP1 Recombinant Antibody (HPAB-0488-YJ) | FC, ELISA | Chimeric (mouse/human) IgG1 |
HPAB-1208WJ | Mouse Anti-STEAP1 Recombinant Antibody (HPAB-1208WJ) | FC, Inhib | Mouse IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MHC-CN0339 | APC-H-2Db/Human STEAP (VSKINRTEM) MHC Tetramer | FCM | |
MHC-CN0340 | PE-H-2Db/Human STEAP (VSKINRTEM) MHC Tetramer | FCM | |
MHC-CN1233 | PE-A*02:01/Human STEAP1 (MLAVFLPIV) MHC Tetramer | FCM, IHC | |
MHC-CN1234 | APC-A*02:01/Human STEAP1 (MLAVFLPIV) MHC Tetramer | FCM, IHC | |
MHC-CN1235 | FITC-A*02:01/Human STEAP1 (MLAVFLPIV) MHC Tetramer | FCM, IHC |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-N0259-YC-S(P) | Human Anti-STEAP1 Recombinant Antibody; scFv Fragment (HPAB-N0259-YC-S(P)) | FuncS | Humanized scFv |
HPAB-0487-YJ-S(P) | Mouse Anti-STEAP1 Recombinant Antibody; scFv Fragment (HPAB-0487-YJ-S(P)) | FC, ELISA | Mouse scFv |
HPAB-0488-YJ-S(P) | Mouse Anti-STEAP1 Recombinant Antibody; scFv Fragment (HPAB-0488-YJ-S(P)) | FC, ELISA | Mouse scFv |
HPAB-0489-YJ-S(P) | Human Anti-STEAP1 Recombinant Antibody; scFv Fragment (HPAB-0489-YJ-S(P)) | FC, ELISA | Humanized scFv |
HPAB-1208WJ-S(P) | Mouse Anti-STEAP1 Recombinant Antibody; scFv Fragment (HPAB-1208WJ-S(P)) | ELISA | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-N0259-YC-F(E) | Human Anti-STEAP1 Recombinant Antibody; Fab Fragment (HPAB-N0259-YC-F(E)) | FuncS | Humanized Fab |
HPAB-0487-YJ-F(E) | Mouse Anti-STEAP1 Recombinant Antibody; Fab Fragment (HPAB-0487-YJ-F(E)) | FC, ELISA | Mouse Fab |
HPAB-0488-YJ-F(E) | Human Anti-STEAP1 Recombinant Antibody; Fab Fragment (HPAB-0488-YJ-F(E)) | FC, ELISA | Fab |
HPAB-0489-YJ-F(E) | Human Anti-STEAP1 Recombinant Antibody; Fab Fragment (HPAB-0489-YJ-F(E)) | FC, ELISA | Humanized Fab |
HPAB-1208WJ-F(E) | Mouse Anti-STEAP1 Recombinant Antibody; Fab Fragment (HPAB-1208WJ-F(E)) | ELISA | Mouse Fab |
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