Mouse Anti-TFRC Recombinant Antibody (VS3-CJ313) (CAT#: VS3-CJ313)

• Immunogen
• KLH-conjugated synthetic peptide between 649-677 amino acids from human TFRC

• Host Species
• Mouse

• Type
• Mouse IgM

• Specificity
• Human TFRC

• Species Reactivity
• Human

• Applications
• WB

• Conjugate
• Unconjugated

• Purification
• Affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Buffer
• PBS

• Preservative
• 0.09% sodium azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• This antibody has been tested for use in Western Blot (1:1000).

• Alternative Names
• T9; TR; TFR; p90; CD71; TFR1; TRFR; IMD46

• Gene ID
• 7037

• UniProt ID
• P02786

• Sequence Similarities
• Belongs to the peptidase M28 family. M28B subfamily.

• Cellular Localization
• Cell membrane, Membrane, Secreted

• Post Translation Modifications
• Stearoylated by ZDHHC6 which inhibits TFRC-mediated activation of the JNK pathway and promotes mitochondrial fragmentation (PubMed:26214738).
  Stearoylation does not affect iron uptake (PubMed:26214738).
  N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
  Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
  Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.

• Protein Refseq
• NP_001121620.1; NP_001300894.1; NP_001300895.1

• Function
• Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes (PubMed:26214738).
  Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity).
  A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
  Acts as a lipid sensor that regulates mitochondrial fusion by regulating activation of the JNK pathway (PubMed:26214738).
  When dietary levels of stearate (C18:0) are low, promotes activation of the JNK pathway, resulting in HUWE1-mediated ubiquitination and subsequent degradation of the mitofusin MFN2 and inhibition of mitochondrial fusion (PubMed:26214738).
  When dietary levels of stearate (C18:0) are high, TFRC stearoylation inhibits activation of the JNK pathway and thus degradation of the mitofusin MFN2 (PubMed:26214738).

Secondary Antibody

• 2ND-052CQ
• Goat Anti-Mouse IgM (H&L) Antibody

• 2RD-A1568-YJ
• Rabbit Anti-Mouse IgM Secondary antibody (Clone RM109)

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS