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  • Rabbit Anti-TSC2 Recombinant Antibody (VS3-CJ997)

Rabbit Anti-TSC2 Recombinant Antibody (VS3-CJ997) (CAT#: VS3-CJ997)

This product is a rabbit antibody that recognizes human, mouse, and rat TSC2.


See more Engineered Antibody products for TSC2
Specific Inquiry
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  • Conjugation:
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  • Purity:
  • Fc Engineering:
  • Gene Expression
  • Datasheet
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  • COA
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30409/if_selected.jpg
Figure 2 IF staining of human cell line HeLa
Figure 2 IF staining of human cell line HeLa
(Immunofluorescent staining of human cell line HeLa shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49679/if_selected.jpg
Figure 3 IF staining of human cell line U-251 MG
Figure 3 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2225/710_C8_6_selected.jpg
Figure 4 IHC staining of human cerebral cortex
Figure 4 IHC staining of human cerebral cortex
(Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30409/62646_B_7_5_selected.jpg
Figure 5 IF staining of human cell line A-431
Figure 5 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30409/335_A8_1_blue_red_green.jpg
Normal Tissue
Figure 6 Cerebral cortex
Figure 6 Cerebral cortex
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30409/62646_B_8_5.jpg
Figure 7 Colon
Figure 7 Colon
(Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30409/62646_A_9_3.jpg
Figure 8 Liver
Figure 8 Liver
(Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30409/62646_A_8_4.jpg
Figure 9 Kidney
Figure 9 Kidney
(Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30409/62646_A_8_5.jpg
Figure 10 Testis
Figure 10 Testis
(Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30409/62646_A_4_6.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Figure 11 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* https://v21.proteinatlas.org/ENSG00000103197-TSC2
Figure 11 RNA cell line category: Low cell line specificity
Figure 11 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000103197-TSC2

Specifications

  • Immunogen
  • Recombinant protein
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse, Rat TSC2
  • Species Reactivity
  • Human, Mouse, Rat
  • Applications
  • WB, IHC, FC
  • Conjugate
  • Unconjugated

Product Property

  • Purification
  • Protein A affinity purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Format
  • Liquid
  • Buffer
  • 40% Glycerol, 1% BSA, TBS, pH7.4.
  • Preservative
  • 0.05% Sodium Azide
  • Storage
  • Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • This antibody has been tested for use in Western Blot, Immunohistochemistry, Flow Cytometry.

Target

  • Alternative Names
  • LAM; TSC4; PPP1R160
  • Cellular Localization
  • Cytoplasm, Membrane
  • Post Translation Modifications
  • Phosphorylation at Ser-1387, Ser-1418 or Ser-1420 does not affect interaction with TSC1. Phosphorylation at Ser-939 and Thr-1462 by PKB/AKT1 is induced by growth factor stimulation. Phosphorylation by AMPK activates it and leads to negative regulation of the mTORC1 complex. Phosphorylated at Ser-1798 by RPS6KA1; phosphorylation inhibits TSC2 ability to suppress mTORC1 signaling. Phosphorylated by DAPK1.
    Ubiquitinated by the DCX(FBXW5) E3 ubiquitin-protein ligase complex, leading to its subsequent degradation. Ubiquitinated by MYCBP2 independently of its phosphorylation status leading to subsequent degradation; association with TSC1 protects from ubiquitination.
  • Function
  • In complex with TSC1, this tumor suppressor inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling (PubMed:12271141, PubMed:28215400).
    Acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1 (PubMed:15340059).
    May also play a role in microtubule-mediated protein transport (By similarity).
    Also stimulates the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 (By similarity).

Recommended Products

Isotype Control

Secondary Antibody

Related Resources

  • Related Signaling Pathways
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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "TSC2"

Recombinant Antibody

CAT Product Name Application Type
DrMAB-636 Human Anti-TSC2 Recombinant Antibody (clone D542) ELISA, IP-MS Human IgG
MRO-1538-CN Rabbit Anti-TSC2 Recombinant Antibody (clone CBACN-564) WB, IHC, FC Rabbit IgG
MRO-2245-CN Rabbit Anti-TSC2 Polyclonal Antibody (MRO-2245-CN) IF, IHC, FC Rabbit IgG
VS3-FY1490 Recombinant Rabbit Anti-TSC2 Antibody (clone R06-0D3) WB, IHC-P, IP Rabbit IgG
VS3-FY2098 Rabbit Anti-TSC2 Recombinant Antibody (clone R04-1I3) WB, IP Rabbit IgG

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-3701 Rabbit Anti-TSC2 Recombinant Antibody (clone DS3701AB) ICC, IF, IHC-P, WB Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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