Mouse Anti-NBN Recombinant Antibody (clone 7E4A2) (CAT#: VS3-XY1135)
Clone 7E4A2 is a mouse antibody, which reacts with human NBN protein. This antibody is suitable for NBN detection by ELISA, WB, IHC, FC, ICC assays.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1429/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1429/if_selected.jpg
Figure 2 IHC staining of human testis
(Immunohistochemical staining of human testis shows strong nuclear positivity in cells of seminiferous ducts and Leydig cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1429/4888_A_4_6_selected.jpg
(Immunohistochemical staining of human testis shows strong nuclear positivity in cells of seminiferous ducts and Leydig cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1429/4888_A_4_6_selected.jpg
Figure 3 Cerebral cortex
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1429/4996_B_9_5.jpg
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1429/4996_B_9_5.jpg
Figure 4 Colon
(Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1429/4888_A_9_3.jpg
(Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1429/4888_A_9_3.jpg
Figure 5 Liver
(Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1429/4888_A_9_4.jpg
(Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1429/4888_A_9_4.jpg
Figure 6 Kidney
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1429/4888_A_7_5.jpg
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1429/4888_A_7_5.jpg
Figure 7 Cervix
(Glandular cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1429/4996_B_7_3.jpg
(Glandular cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1429/4996_B_7_3.jpg
Figure 8 Lymph node
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1429/4888_A_7_8.jpg
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1429/4888_A_7_8.jpg
Figure 9 RNA cell line category: Cell line enhanced (SK-BR-3)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000104320-NBN
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000104320-NBN
Specifications
- Immunogen
- Recombinant fragment of human NBN (a.a. 467-615) expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human NBN
- Species Reactivity
- Human
- Clone
- 7E4A2
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000), Immunohistochemistry (1/200-1/1000), Flow Cytometry (1/200-1/400) and Immunocytochemistry (1/200-1/1000).
Target
- Alternative Names
- Nibrin; Nijmegen Breakage Syndrome 1 (Nibrin); Cell Cycle Regulatory Protein P95; NBS1; NBS; P95
- Gene ID
- 4683
- UniProt ID
- O60934
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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|---|---|---|---|
| MOB-692 | Recombinant Anti-human NBN Antibody | WB, IHC, FuncS | IgG |
| MHH-692 | Recombinant Human Anti-human NBN Antibody | FC, WB, FuncS | IgG |
| MRO-1159-CN | Recombinant Rabbit Anti-NBN Monoclonal Antibody (CBACN-422) | WB, IF, IP, FC, IHC, ChIP | Rabbit IgG |
| ZG-0481U | Rabbit Anti-Phospho-NBN (S343) Recombinant Antibody (clone 1B4) | ELISA, WB | Rabbit IgG |
| VS3-CJ165 | Rabbit Anti-NBN Recombinant Antibody (VS3-CJ165) | WB, ICC, IF, IHC, IP | Rabbit IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-692-F(E) | Recombinant Anti-human NBN Antibody Fab Fragment | IP, RIA, FuncS | Fab |
| MHH-692-F(E) | Recombinant Human Anti-human NBN Antibody Fab Fragment | IF, FC, FuncS | Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-692-S(P) | Recombinant Anti-human NBN Antibody scFv Fragment | WB, IF, FuncS | scFv |
| MHH-692-S(P) | Recombinant Human Anti-human NBN Antibody scFv Fragment | WB, ELISA, IHC, FuncS | scFv |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2371 | Hi-Affi™ Recombinant Rabbit Anti-NBN Monoclonal Antibody (DS2371AB) | ICC, IP, WB | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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