Recombinant Rabbit Anti-FGF2 Detection Antibody (clone R06-5J6) (CAT#: VS3-WK1677)
This antibody is a rabbit recombinant antibody against human FGF2. It was developed by immunization of rabbit with recombinant human FGF2 protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. R02-6J3 and R06-5J6 were identified as antibody pair for sandwich ELISA. As a detection antibody, the clone R06-5J6 can be used in ELISA analysis to identify human FGF2.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear bodies.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/641_G7_4_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear bodies.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/641_G7_4_selected.jpg
Figure 3 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear bodies.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/640_G7_1_red_green.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear bodies.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/640_G7_1_red_green.jpg
Figure 4 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/642_G7_1_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear bodies.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/642_G7_1_red_green.jpg
Figure 2 IHC staining of human caudate
(Immunohistochemical staining of human caudate shows nuclear positivity in glia.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/ih_additional_tissue/65502/1336_684_200x200.jpg
(Immunohistochemical staining of human caudate shows nuclear positivity in glia.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/ih_additional_tissue/65502/1336_684_200x200.jpg
Figure 5 Cerebral cortex
(Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/2074_B_9_5.jpg
(Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/2074_B_9_5.jpg
Figure 6 Caudate
(Glial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/2074_B_8_7.jpg
(Glial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/2074_B_8_7.jpg
Figure 7 Colon
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/2074_A_8_3.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/2074_A_8_3.jpg
Figure 8 Kidney
(Cells in glomeruli
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/2074_A_9_5.jpg
(Cells in glomeruli
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/125/2074_A_9_5.jpg
Figure 9 RNA cell line category: Cell line enhanced (BJ, BJ hTERT+, fHDF/TERT166, hTERT-HME1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000138685-FGF2
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000138685-FGF2
Specifications
- Immunogen
- Recombinant human FGF2 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human FGF2
- Species Reactivity
- Human
- Clone
- R06-5J6
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01M PBS, pH 7.2
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- Fibroblast Growth Factor 2; Fibroblast Growth Factor 2 (Basic); Heparin-Binding Growth Factor 2; HBGF-2; FGF-2; BFGF
- Gene ID
- 2247
- UniProt ID
- P09038
- Long Name
- Fibroblast Growth Factor 2
- Sequence Similarities
- Belongs to the heparin-binding growth factors family.
- Cellular Localization
- Nucleus, Secreted
- Post Translation Modifications
- Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.
- Protein Refseq
- NP_001997.5
- Function
- Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4. Also acts as an integrin ligand which is required for FGF2 signaling. Binds to integrin ITGAV:ITGB3.
Recommended Products
Capture antibody
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "FGF2"
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-357-F(E) | Recombinant Anti-human FGF2 Antibody Fab Fragment | IF, FC, FuncS | Fab |
| MHH-357-F(E) | Recombinant Human Anti-human FGF2 Antibody Fab Fragment | IP, IF, Biosensors, FuncS | Fab |
| HPAB-0164-WJ-F(E) | Mouse Anti-FGF2 Recombinant Antibody (clone E12); Fab Fragment | ELISA | Mouse Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-357-S(P) | Recombinant Anti-human FGF2 Antibody scFv Fragment | ELISA, WB, FC, FuncS | scFv |
| MHH-357-S(P) | Recombinant Human Anti-human FGF2 Antibody scFv Fragment | ELISA, WB, IP, FuncS | scFv |
| HPAB-0164-WJ-S(P) | Mouse Anti-FGF2 Recombinant Antibody (clone E12); scFv Fragment | ELISA | Mouse scFv |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-279CL | Human Anti-FGF2 Recombinant Antibody (TAB-279CL) | ELISA | Human IgG |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-048WM | Anti-Human FGF2 Recombinant Antibody (3F12E7) | ELISA, WB, IHC, FuncS | |
| TAB-048WM-S(P) | Anti-Human FGF2 Recombinant Antibody scFv Fragment (3F12E7) | ELISA, FuncS | |
| TAB-048WM-F(E) | Anti-Human FGF2 Recombinant Antibody Fab Fragment (3F12E7) | ELISA, FuncS |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-049WM | Anti-Human FGF2 Recombinant Antibody (6) | ELISA, WB | Human Antibody |
| TAB-049WM-S(P) | Anti-Human FGF2 Recombinant Antibody scFv Fragment (6) | ELISA, WB | Human Antibody |
| TAB-049WM-F(E) | Anti-Human FGF2 Recombinant Antibody Fab Fragment (6) | ELISA, WB | Human Antibody |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0725CT | Recombinant Mouse anti-Human FGF2 Monoclonal antibody (3D9) | ELISA | |
| MRO-0576-CN | Rabbit Anti-FGF2 Recombinant Antibody (clone JM28-10) | WB, IP, FC | Rabbit IgG |
| ZG-0343J | Mouse Anti-FGF2 Recombinant Antibody (ZG-0343J) | WB, IHC, ELISA | Mouse IgG |
| VS3-XY701 | Mouse Anti-FGF2 Recombinant Antibody (clone 2H5G2C11) | ELISA, WB, IHC | |
| VS3-WK1347 | Mouse Anti-FGF2 Recombinant Antibody (VS3-WK1347) | WB, FC, IP | Mouse IgG |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-800CQ | Mouse Anti-FGF2 Recombinant Antibody (clone 0.T.50) | Neut | Mouse IgG1, κ |
| NEUT-801CQ | Mouse Anti-FGF2 Recombinant Antibody (clone CBL979) | Neut | Mouse IgG1, κ |
| NEUT-802CQ | Recombinant Mouse Anti-FGF2 Antibody (FB-8) | RIA, ELISA, Dot, Neut, WB, IHC-FoFr | IgG1 |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1293 | Hi-Affi™ Rabbit Anti-FGF2 Recombinant Antibody (clone DS1293AB) | FC, IP, WB | Rabbit IgG |
Neuroscience Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-009CN | Human Anti-FGF2 Recombinant Antibody (clone YC055) | ELISA | Humanized IgG |
| NS-026CN | Mouse Anti-FGF2 Recombinant Antibody (clone Dan-2) | ELISA, FC | Mouse IgG |
| NS-009CN-F(E) | Human Anti-FGF2 Recombinant Antibody (clone YC056); Fab Fragment | ELISA | Humanized Fab |
| NS-026CN-F(E) | Mouse Anti-FGF2 Recombinant Antibody (clone YC058); Fab Fragment | ELISA, FC | Mouse Fab |
| NS-009CN-S(P) | Human Anti-FGF2 Recombinant Antibody (clone YC054); scFv Fragment | ELISA | Humanized scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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