Recombinant Rabbit Anti-ITGA5 Antibody (clone R02-3G4) (CAT#: VS3-FY770)

• Immunogen
• Synthetic peptide of human integrin α5.

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse ITGA5

• Species Reactivity
• Human, Mouse

• Clone
• R02-3G4

• Applications
• Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation

• Conjugate
• Unconjugated

• MW
• Calculated MW: 115 kDa; Observed MW: 150 kDa

• Purification
• Affinity Purified

• Purity
• >95% as determined by SDS-PAGE

• Buffer
• 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA

• Preservative
• 0.01% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunohistochemistry-Paraffin: 1/50-1/100
  Immunoprecipitation: 1/20

• Alternative Names
• Integrin Subunit Alpha 5

• Gene ID
• 3678

• UniProt ID
• P08648

• Sequence Similarities
• Belongs to the integrin alpha chain family.

• Cellular Localization
• Cell junction, Membrane

• Post Translation Modifications
• Proteolytic cleavage by PCSK5 mediates activation of the precursor.

• Protein Refseq
• NP_002196.4

• Function
• Integrin alpha-5/beta-1 (ITGA5:ITGB1) is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. ITGA5:ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877).
  ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887, PubMed:17158881).
  ITGA5:ITGB1 is a receptor for IL1B and binding is essential for IL1B signaling (PubMed:29030430).
  ITGA5:ITGB3 is a receptor for soluble CD40LG and is required for CD40/CD40LG signaling (PubMed:31331973).
  (Microbial infection) Integrin ITGA5:ITGB1 acts as a receptor for Human metapneumovirus.
  (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human parvovirus B19.
  (Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody

• 2ND-057CQ
• Donkey Anti-Rabbit IgG (H&L) Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-060CQ
• Llama Anti-Rabbit IgG (H&L) Antibody

• 2ND-061CQ
• Sheep Anti-Rabbit IgG (H&L) Antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody