Recombinant Rabbit Anti-PDHA1 Antibody (clone R06-1J4) (CAT#: VS3-FY1118)

This product is a recombinant rabbit antibody that recognizes PDHA1. This antibody has been reported for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R06-1J4 is specific for human, mouse, rat PDHA1. The antigen is synthetic peptide of human pyruvate dehydrogenase e1-α subunit.


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WB

Figure 1. Recombinant Rabbit Anti-PDHA1 Antibody (clone R06-1J4) in WB.

Figure 1. Recombinant Rabbit Anti-PDHA1 Antibody (clone R06-1J4) in WB.

Western Blot analysis of pyruvate dehydrogenase E1alpha subunit in C6, 3T3 lysates using Pyruvate Dehydrogenase E1 Alpha Antibody.

ICC

Figure 2. Recombinant Rabbit Anti-PDHA1 Antibody (clone R06-1J4) in ICC.

Figure 2. Recombinant Rabbit Anti-PDHA1 Antibody (clone R06-1J4) in ICC.

Immunocytochemical analysis of pyruvate dehydrogenase E1alpha subunit (green) in Hela using pyruvate dehydrogenase E1alpha subunit antibody and DAPI (blue).


Specifications

  • Immunogen
  • Synthetic Peptide of Human Pyruvate Dehydrogenase E1-α Subunit.
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse, Rat PDHA1
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • R06-1J4
  • Applications
  • Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
  • Conjugate
  • Unconjugated
  • MW
  • Calculated MW: 43 kDa; Observed MW: 43 kDa

Product Property

  • Purification
  • Affinity Purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Buffer
  • 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
  • Preservative
  • 0.01% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunofluorescence: 1/50-1/200
    Immunoprecipitation: 1/20

Target

  • Alternative Names
  • Pyruvate Dehydrogenase E1 Alpha 1 Subunit; Pyruvate Dehydrogenase E1 Component Subunit Alpha, Somatic Form, Mitochondrial; Pyruvate Dehydrogenase (Lipoamide) Alpha 1; Pyruvate Dehydrogenase Alpha 1; PDHE1-A Type I; EC 1.2.4.1; PHE1A

Recommended Products

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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