Recombinant Mouse Anti-PPARG Antibody (VS4-CJ84) (CAT#: VS4-CJ84)

• Immunogen
• Recombinant fragment, corresponding to amino acids 254-478aa of human PPARG (C-terminus).

• Host Species
• Mouse

• Type
• Mouse IgG1

• Specificity
• Human, Dog, Porcine, Rat, Mouse PPARG

• Species Reactivity
• Human, Dog, Porcine, Rat, Mouse

• Applications
• ELISA, WB, ICC

• Conjugate
• Unconjugated

• Purification
• Affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• 50% Glycerol, 0.1M Tris-Glycine (150 mM NaCl), pH 7.4.

• Preservative
• 0.2% Sodium Azide

• Storage
• Store at -20°C or -80°C upon receipt. Avoid repeated freeze.

• Application Notes
• This antibody has been tested for use in ELISA, Western Blot (1:500-1:5000), Immunocytochemistry (1:50-1:200).

• Alternative Names
• GLM1; CIMT1; NR1C3; PPARG1; PPARG2; PPARG5; PPARgamma

• Gene ID
• 5468

• UniProt ID
• P37231

• Long Name
• Peroxisome proliferator activated receptor gamma

• Sequence Similarities
• Belongs to the nuclear hormone receptor family. NR1 subfamily.

• Cellular Localization
• Cytoplasm, Nucleus

• Post Translation Modifications
• O-GlcNAcylation at Thr-84 reduces transcriptional activity in adipocytes.
  Phosphorylated in basal conditions and dephosphorylated when treated with the ligand. May be dephosphorylated by PPP5C. The phosphorylated form may be inactive and dephosphorylation at Ser-112 induces adipogenic activity (By similarity).
  Ubiquitinated by E3 ubiquitin-protein ligase complex containing FBXO9; leading to proteasomal degradation.

• Protein Refseq
• NP_005028.4; NP_056953.2; NP_619725.2

• Function
• Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated pro-inflammatory responses. Plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of BMAL1 in the blood vessels (By similarity).
  (Microbial infection) Upon treatment with M.tuberculosis or its lipoprotein LpqH, phosphorylation of MAPK p38 and IL-6 production are modulated, probably via this protein.

Isotype Control

• MOB-065CQ
• Mouse IgG1 Isotype Control Antibody

Secondary Antibody

• 2RD-A1562-YJ
• Rabbit Anti-Mouse IgG1 Secondary antibody (Clone RM106)

• 2RD-A1460-YJ
• Goat Anti-Mouse IgG1 Secondary antibody