Recombinant Rabbit Anti-VCAM1 Antibody (clone R02-7H1) (CAT#: VS3-FY1539)

This product is a recombinant rabbit antibody that recognizes VCAM1. This antibody has been reported for use in Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation. The clone R02-7H1 is specific for human, mouse, rat VCAM1. The antigen is synthetic peptide of vcam1.


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WB

Figure 1. Recombinant Rabbit Anti-VCAM1 Antibody (clone R02-7H1) in WB.

Figure 1. Recombinant Rabbit Anti-VCAM1 Antibody (clone R02-7H1) in WB.

Western Blot analysis of VCAM1 in 3T3 lysates using VCAM1 antibody.


Specifications

  • Immunogen
  • Synthetic peptide of VCAM1.
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse, Rat VCAM1
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • R02-7H1
  • Applications
  • Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
  • Conjugate
  • Unconjugated
  • MW
  • Calculated MW: 81 kDa; Observed MW: 110 kDa

Product Property

  • Purification
  • Affinity Purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Buffer
  • 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
  • Preservative
  • 0.01% Sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunohistochemistry-Paraffin: 1/50-1/100
    Immunoprecipitation: 1/20

Target

  • Alternative Names
  • Vascular Cell Adhesion Molecule 1; CD106 Antigen; INCAM-100; Vascular Cell Adhesion Protein 1; V-CAM 1; VCAM-1; CD106
  • Cellular Localization
  • Membrane
  • Post Translation Modifications
  • Sialoglycoprotein.
  • Function
  • Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.

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Related Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "VCAM1"

Recombinant Antibody

Single-domain Antibody

CAT Product Name Application Type
NABL-061 Recombinant Anti-Human VCAM1 VHH Single Domain Antibody WB, ELISA, IP, FuncS Llama VHH

Intrabody

CAT Product Name Application Type
IAB-B067(A) Recombinant Anti-human VCAM-1 Intrabody [(D-Arg)9] FACS, ELISA, IF, FuncS scFv-(D-Arg)9
IAB-B067(G) Recombinant Anti-human VCAM-1 Intrabody [+36 GFP] FACS, ICC, FuncS scFv-(+36GFP)
IAB-B067(T) Recombinant Anti-human VCAM-1 Intrabody [Tat] PCA, WB, FuncS scFv-Tat

Human Antibody

Blocking Antibody

Neutralizing Antibody

CAT Product Name Application Type
NEUT-2250CQ Rat Anti-Vcam1 Recombinant Antibody (clone mAb0829) Neut, FC, IHC, IHC-Fr, IP, CyTOF Rat IgG1
NEUT-2253CQ Mouse Anti-VCAM1 Recombinant Antibody (clone 1.G11B1) WB, Neut, ELISA, FC, IHC, IHC-Fr, IP, ICC Mouse IgG1

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-3829 Rabbit Anti-VCAM1 Recombinant Antibody (clone DS3829AB) WB, ICC, IP Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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