Recombinant Mouse Anti-WT1 Antibody (clone 7F9E8) (CAT#: VS3-FY1556)

• Host Species
• Mouse

• Type
• Mouse IgG1

• Clone
• 7F9E8

• Applications
• Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Flow Cytometry

• Conjugate
• Unconjugated

• Purity
• >95% as determined by SDS-PAGE

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Flow Cytometry.

• Alternative Names
• Wilms Tumor 1; WT33; Wilms Tumor Protein; NPHS4; WIT-2; WAGR; AWT1; GUD

• Gene ID
• 7490

• UniProt ID
• P19544

• Sequence Similarities
• Belongs to the EGR C2H2-type zinc-finger protein family.

• Cellular Localization
• Cytoplasm, Nucleus

• Protein Refseq
• NP_000369.3; NP_001185480.1; NP_001185481.1

• Function
• Transcription factor that plays an important role in cellular development and cell survival (PubMed:7862533).
  Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3' (PubMed:7862533, PubMed:17716689, PubMed:25258363).
  Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors (PubMed:15520190).
  Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing (PubMed:16934801).
  Isoform 1 has lower affinity for DNA, and can bind RNA (PubMed:19123921).

Secondary Antibody

• 2ND-047CQ
• Goat Anti-Mouse IgG1 Antibody, R-PE

• 2RD-A1460-YJ
• Goat Anti-Mouse IgG1 Secondary antibody

Isotype Control

• MOB-065CQ
• Mouse IgG1 Isotype Control Antibody