This is an antibody that binds to human complement component C5, which inhibits cleavage of human complement component C5 into C5a and C5b. Antibody m5G1.1 scFv could completely block complement hemolytic activity as well as the generation of sC5b-9.
Figure 1 Inhibition of 5G1.1 mAb binding to human C5 by recombinant humanized h5G1.1 HuG4 mAb.
Biotinylated 5G1. 1 mAb (0.2 nM), plus the indicated concentrations of unlabeled murine 5G1.1 mAb, recombinant h5G1.1 HuG4 mAb or a negative control recombinant HuG4 mAb (ch3F4 HuG4 mAb), were incubated together for 2 hr at 37°C in microtiter plates coated with human C5. After washing, bound biotinylated 5G1. 1 mAb was detected using peroxidase-conjugated streptavidin, with quantitation performed by monitoring hydrolysis of o-phenylenediamine dihydrochloride at 490 nm. Absorbance values obtained in the presence of recombinant inhibitor were normalized in each individual experiment, to the value obtained in the absence of competitor. Results shown are mean values of four determinations.
Thomas, T. C., Rollins, S. A., Rother, R. P., Giannoni, M. A., Hartman, S. L., Elliott, E. A., ... & Evans, M. J. (1996). Inhibition of complement activity by humanized anti-C5 antibody and single-chain Fv. Molecular immunology, 33(17-18), 1389-1401.
Figure 2 Protection of porcine aortic endothelial cells from human serum complement by h5G1.1 HuG4 mAb.
Calceinlabeled PAEC were incubated with HBSS containing 1% BSA and 0.85 mg/ml purified rabbit anti-PAEC polyclonal antibody for 30min at 37°C. The cells were washed and incubated with HBSS containing 1% BSA plus 20% human serum and the indicated concentrations of inhibitor for 30 min at 37°C. Calcein dye release and retention were detemined by measurement of the fluorescence of supernatants and SDS-solubilized cells, respectively, with dye release in the absence of human serum subtracted from all values. Results shown are the mean of three independent determinations. The arrow denotes the predicted concentration of hC5 in 20% serum.
Thomas, T. C., Rollins, S. A., Rother, R. P., Giannoni, M. A., Hartman, S. L., Elliott, E. A., ... & Evans, M. J. (1996). Inhibition of complement activity by humanized anti-C5 antibody and single-chain Fv. Molecular immunology, 33(17-18), 1389-1401.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
CAT | Product Name | Application | Type |
---|---|---|---|
DrMAB-934 | Recombinant Human Anti-C5 Antibody scFv Fragment | ELISA, SPR, Inhib | scFv |
HPAB-0610-CN-S(P) | Mouse Anti-C5 Recombinant Antibody; scFv Fragment (HPAB-0610-CN-S(P)) | ELISA, Block | Mouse scFv |
HPAB-0123-LS-S(P) | Mouse Anti-C5 Recombinant Antibody; scFv Fragment (HPAB-0123-LS-S(P)) | Block, ELISA | Mouse scFv |
HPAB-0124-LS-S(P) | Mouse Anti-C5 Recombinant Antibody; scFv Fragment (HPAB-0124-LS-S(P)) | Block, ELISA | Mouse scFv |
HPAB-0080-WJ-S(P) | Human Anti-C5 Recombinant Antibody; scFv Fragment (HPAB-0080-WJ-S(P)) | ELISA, FC | Humanized scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-1366CL | Mouse Anti-C5 Recombinant Antibody (TAB-1366CL) | ELISA, Inhib | Mouse IgG |
TAB-1367CL | Mouse Anti-C5 Recombinant Antibody (TAB-1367CL) | ELISA, Inhib | Mouse IgG |
TAB-1368CL | Mouse Anti-C5 Recombinant Antibody (TAB-1368CL) | ELISA | Mouse IgG |
TAB-1370CL-S(P) | Mouse Anti-C5 Recombinant Antibody; scFv Fragment (TAB-1370CL-S(P)) | ELISA | Mouse scFv |
TAB-1371CL-S(P) | Mouse Anti-C5 Recombinant Antibody; scFv Fragment (TAB-1371CL-S(P)) | Inhib, FuncS | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-1372CL | Human Anti-C5 Recombinant Antibody (TAB-1372CL) | Inhib, FuncS | Humanized antibody |
TAB-1373CL | Human Anti-C5 Recombinant Antibody (TAB-1373CL) | IB, CDC, Inhib | Humanized IgG |
TAB-1374CL | Human Anti-C5 Recombinant Antibody (TAB-1374CL) | ELISA, CDC, Inhib | Humanized IgG |
TAB-1375CL | Human Anti-C5 Recombinant Antibody (TAB-1375CL) | CDC, Inhib | Humanized IgG |
TAB-1376CL | Human Anti-C5 Recombinant Antibody (TAB-1376CL) | CDC, Inhib | Humanized IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-082CQ | Mouse Anti-C5 Recombinant Antibody (clone CBL249) | ELISA, Neut | Mouse IgG2a |
NEUT-083CQ | Mouse Anti-C5 Recombinant Antibody (clone MM0608-6H13) | ELISA, Neut, WB | Mouse IgG1 |
NEUT-084CQ | Mouse Anti-C5 Recombinant Antibody (clone CBL030) | ELISA, WB, Neut | Mouse IgG1 |
NEUT-085CQ | Mouse Anti-C5 Recombinant Antibody (clone CBL250) | ELISA, WB, IHC, FuncS | Mouse IgG1 |
NEUT-086CQ | Mouse Anti-C5 Recombinant Antibody (clone G25/2) | ELISA, IP, WB, Neut | Mouse IgG1, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-088CQ | Mouse Anti-C5 Recombinant Antibody (clone CBL771) | WB, Block, ELISA | Mouse IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-4104 | Hi-Affi™ Rabbit Anti-C5 Recombinant Antibody (clone SI175DS) | IHC-P | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-453CQ | Anti-Human C5 Recombinant Antibody (Tesidolumab) | ELISA, IHC, FC, IP, IF, FuncS | IgG1, λ2 |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0123-LS-F(E) | Mouse Anti-C5 Recombinant Antibody; Fab Fragment (HPAB-0123-LS-F(E)) | Block, ELISA | Mouse Fab |
HPAB-0124-LS-F(E) | Mouse Anti-C5 Recombinant Antibody; Fab Fragment (HPAB-0124-LS-F(E)) | Block, ELISA | Mouse Fab |
HPAB-0127CQ-F(E) | Human Anti-C5 Recombinant Antibody; Fab Fragment (HPAB-0127CQ-F(E)) | ELISA, WB, Inhib, FuncS | Human Fab |
HPAB-0128CQ-F(E) | Mouse Anti-C5 Recombinant Antibody (clone BB5.1); Fab Fragment | FC, FuncS, IA, IF, IP, IHC | Mouse Fab |
HPAB-0656LY-F(E) | Mouse Anti-C5 Recombinant Antibody; Fab Fragment (HPAB-0656LY-F(E)) | IP, RIA, ELISA, WB | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0652LY | Mouse Anti-C5 Recombinant Antibody (HPAB-0652LY) | IP, RIA, ELISA, WB | Mouse IgG |
HPAB-0653LY | Mouse Anti-C5 Recombinant Antibody (HPAB-0653LY) | IP, RIA, ELISA, WB | Mouse IgG |
HPAB-0654LY | Mouse Anti-C5 Recombinant Antibody (HPAB-0654LY) | IP, RIA, ELISA, WB | Mouse IgG |
HPAB-0655LY | Mouse Anti-C5 Recombinant Antibody (HPAB-0655LY) | IP, RIA, ELISA, WB | Mouse IgG |
HPAB-0656LY | Mouse Anti-C5 Recombinant Antibody (HPAB-0656LY) | IP, RIA, ELISA, WB | Mouse IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
PABC-559 | Recombinant Llama Anti-C5 Single Domain Antibody (Nb36) | ELISA | Llama VHH |
CAT | Product Name | Application | Type |
---|---|---|---|
AFC-TAB-453CQ | Afuco™ Anti-C5 ADCC Recombinant Antibody (Tesidolumab), ADCC Enhanced | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
AFC-TAB-008 | Afuco™ Anti-C5 ADCC Recombinant Antibody (Eculizumab), ADCC Enhanced | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
AFC-TAB-436CQ | Afuco™ Anti-C5 ADCC Recombinant Antibody (Olendalizumab), ADCC Enhanced | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
There are currently no Customer reviews or questions for HPAB-0614-CN. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.