Recombinant Human Antibody (EP6b-B01) is capable of binding to TNFR 6, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-TNFR 6 mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-TNFR 6 proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. This antibody could efficiently trigger apoptosis and therefore function as a potent agonist.
Figure 1 Kinetic explanation of Fas agonist activity.
Jurkat cells were incubated with the selected antibodies or FasL for the indicated time points. Agonistic activity was monitored by caspase substrate z-DEVD cleavage. ( a ) Experiment performed without cross-linking the antibodies, showing the rate of caspase induction over time. Also labelled next to the relevant curve is the rate of k off (s À 1 ) for each antibody, as determined by SPR. ( b ) The same experiment was performed following cross-linking of the antibodies with protein A. These experiments were performed in triplicate and error bars indicate the S.D. between replicates. ( c ) Schematic illustration of the difference in agonism between an antibody with relatively fast dissociation from Fas (above) and an antibody with relatively slow dissociation (below). The antibody with the faster rate of k off (above) is better able to recruit new Fas monomers and create an active signalling complex.
Chodorge, M., Züger, S., Stirnimann, C., Briand, C., Jermutus, L., Grütter, M. G., & Minter, R. R. (2012). A series of Fas receptor agonist antibodies that demonstrate an inverse correlation between affinity and potency. Cell death and differentiation, 19(7), 1187.
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CAT | Product Name | Application | Type |
---|---|---|---|
PFBL-723 | Human Anti-FAS Recombinant Antibody (clone E09); Fab Fragment | WB, IF, FuncS | Human Fab |
PFBL-724 | Human Anti-FAS Recombinant Antibody (clone EP6b-B01); Fab Fragment | WB, FuncS | Human Fab |
HPAB-0035-YJ-F(E) | Human Anti-FAS Recombinant Antibody (clone h-HFE7A); Fab Fragment | ELISA, Cyt, Apop | Human Fab |
HPAB-0450-YJ-F(E) | Rabbit Anti-FAS Recombinant Antibody; Fab Fragment (HPAB-0450-YJ-F(E)) | ELISA, FC | Rabbit Fab |
HPAB-0451-YJ-F(E) | Rabbit Anti-FAS Recombinant Antibody; Fab Fragment (HPAB-0451-YJ-F(E)) | ELISA, FC | Rabbit Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
PSBL-723 | Human Anti-FAS Recombinant Antibody (clone E09); scFv Fragment | WB, IF, FuncS | Human scFv |
PSBL-724 | Human Anti-FAS Recombinant Antibody (clone EP6b-B01); scFv Fragment | WB, FuncS | Human scFv |
HPAB-0035-YJ-S(P) | Human Anti-FAS Recombinant Antibody (clone h-HFE7A); scFv Fragment | ELISA, Cyt, Apop | Human scFv |
HPAB-1575WJ-S(P) | Mouse Anti-FAS Recombinant Antibody; scFv Fragment (clone F919-9-18) | ELISA, WB | Mouse scFv |
HPAB-1576WJ-S(P) | Human Anti-FAS Recombinant Antibody; scFv Fragment (clone F919) | ELISA, WB | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-029WM | Anti-Human FAS Recombinant Antibody (F45D9-γ1) | FC | Human Antibody |
TAB-029WM-S(P) | Anti-Human FAS Recombinant Antibody scFv Fragment (F45D9-γ1) | IHC, ELISA, FC | Human Antibody |
TAB-029WM-F(E) | Anti-Human FAS Recombinant Antibody Fab Fragment (F45D9-γ1) | IHC, ELISA, FC | Human Antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
BRD-0201MZ | Chicken Anti-FAS (ab1) Polyclonal IgY | WB | Chicken antibody |
BRD-0202MZ | Chicken Anti-FAS (ab2) Polyclonal IgY | WB | Chicken antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-767CQ | Mouse Anti-FAS Recombinant Antibody (clone CBL933) | Neut, FC, WB | Mouse IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-768CQ | Mouse Anti-FAS Recombinant Antibody (clone ZB4) | Block, FC, IHC, WB | Mouse IgG1 |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-1252 | Hi-Affi™ Rabbit Anti-FAS Recombinant Antibody (clone DS1252AB) | WB, ICC | Rabbit IgG |
MOR-1253 | Hi-Affi™ Rabbit Anti-Fas Recombinant Antibody (clone DS1253AB) | ELISA | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-103LC | Mouse Anti-FAS Recombinant Antibody (clone CH11) | FC, ICC, WB |
CAT | Product Name | Application | Type |
---|---|---|---|
MRO-0569-CN | Rabbit Anti-FAS Recombinant Antibody (clone JJ0942) | WB, IF, IHC, FC | Rabbit IgG |
HPAB-0035-YJ | Human Anti-FAS Recombinant Antibody (clone h-HFE7A) | ELISA, Cyt, Apop | Human IgG1, κ |
HPAB-1575WJ | Mouse Anti-FAS Recombinant Antibody (clone F919-9-18) | ELISA, WB | Mouse IgG |
HPAB-1576WJ | Human Anti-FAS Recombinant Antibody (clone F919) | ELISA, WB | Human IgG |
ZG-0711F | Mouse Anti-FAS Recombinant Antibody (clone 3.22) | FC | Mouse IgG1 |
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