Mouse Anti-AQP4 Recombinant Antibody (clone 4H1) (CAT#: ZG-0035J)

Figure 1 Mouse Anti-AQP4 Recombinant Antibody (clone 4H1) in IHC

Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1.Aquaporin 4 Monoclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for Antibody retrieval(>98°C, 20min). 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary Antibody only.

Figure 2 Mouse Anti-AQP4 Recombinant Antibody (clone 4H1) in IF

Immunofluorescence analysis of Human-appendix tissue. 1.Aquaporin 4 Monoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary Antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI.Picture C: merge of A+B

Figure 3 Mouse Anti-AQP4 Recombinant Antibody (clone 4H1) in IF

Immunofluorescence analysis of Mouse-brain tissue. 1.Aquaporin 4 Monoclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary Antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI.Picture C: merge of A+B

Figure 4 Mouse Anti-AQP4 Recombinant Antibody (clone 4H1) in WB

Western blot analysis of 1) Hela, 2) Mouse Heart tissue, 3) Rat Heart Tissue, diluted at 1:2000.

Figure 5 Mouse Anti-AQP4 Recombinant Antibody (clone 4H1) in WB

Western blot analysis of lysates from 1) Hela, 2) Mouse Heart tissue, 3) Rat Heart Tissue cells, (Green) primary Antibody was diluted at 1:1000, 4°C over night, secondary Antibody(AS0811)was diluted at 1:10000, 37°C 1 hour. (Red) GAPDH Polyclonal Antibody Antibody was diluted at 1:5000 as loading control, 4°Cover night, secondary Antibody was diluted at 1:10000, 37°C1 hour.