Mouse Anti-CD40 Recombinant Antibody (clone 2C5) (CAT#: HPAB-0264-YJ)

Figure 1 Flow cytometric assessment of the expression of CD40 on Bu-1 positive primary chicken B-cells (A), MHC-II positive primary chicken macrophages (B), chicken DT40 B-cells (C), and chicken HD11 macrophages (D). color in this figure legend, the reader is referred to the web version of the article.)

Distribution of fluorescence intensities of cells stained with 5 μg of either R-Phycoerythrin-labeled 2C5 (red) or RPhycoerythrin-labeled control mouse IgG1 (blue) per one hundred thousand cells. The yellow histogram represents unstained cells.

Chen, C. H., Abi-Ghanem, D., Njongmeta, L., Bray, J., Mwangi, W., Waghela, S. D.,... & Berghman, L. R. (2010). Production and characterization of agonistic monoclonal antibodies against chicken CD40. Developmental & Comparative Immunology, 34(11), 1139-1143.

Figure 2 Biological effects of anti-chCD40 mAb 2C5.

(A) The vertical axis shows the concentration of nitrite produced in supernatants from chicken HD11 macrophages stimulated by serial three-fold dilutions of purified anti-chCD40 mAb 2C5 (solid squares), negative isotype control mouse IgG1 (solid circles), or negative control PBS (solid triangles). (B) Enhanced proliferation in serum-starved chicken DT40 cells as a function of time. The vertical axis shows the cell density (cells/mL) of live chicken DT40 cells incubated in the presence of 5 μg/mL of purified anti-chCD40 mAb 2C5 (solid squares) or negative control mouse IgG1 (solid circles) after 24, 48, and 72 h. (C) Enhanced proliferation of serum-starved chicken DT40 cells as a function of 2C5 concentration. The vertical axis shows the cell density (cells/mL) of live chicken DT40 cells incubated in the presence of serial three-fold dilutions of 2C5 or control mouse IgG1 for 24, 48, and 72 h. Values are means of triplicate observations ± SD; n = 3; *P < 0.05; **P < 0.01; ***P < 0.001.

Chen, C. H., Abi-Ghanem, D., Njongmeta, L., Bray, J., Mwangi, W., Waghela, S. D.,... & Berghman, L. R. (2010). Production and characterization of agonistic monoclonal antibodies against chicken CD40. Developmental & Comparative Immunology, 34(11), 1139-1143.