The murine Mab 14E1 binds to the extracellular domain of human EGFR. The ability of Mab 14E1 to recognize EGFRvIII which lacks amino acids 6 to 273 of the extracellular domain was tested in immunoblot experiments.
Figure 1 In vitro expression of full-length 14E1 and 14E1A monoclonal antibody.
(a) Western blot analysis of supernatants from transiently transfected 293F cells expressing 14E1 and 14E1A antibody. Supernatant from 293F cells transfected with an empty pZAC2.1 vector (EV) plasmid served as a negative control. The samples were separated by SDS-polyacrylamide gel electrophoresis (PAGE) under (a) nonreducing and (b) reducing conditions and subsequently probed with a goat anti-mouse immunoglobulin G (IgG;H+L) polyclonal antibody. Under reducing conditions, HC refers to the heavy chain and LC refers to the light chain.
Ho, D. T., Wykoff-Clary, S., Gross, C. S., Schneider, D., Jin, F., Kretschmer, P. J., & Hermiston, T. W. (2009). Growth inhibition of an established A431 xenograft tumor by a full-length anti-EGFR antibody following gene delivery by AAV. Cancer gene therapy, 16(2), 184-194.
Figure 2 14E1 monoclonal antibody inhibits the in vitro cell proliferation of A431 cells.
The 14E1 antibody significantly inhibited the proliferation of the A431 cells in a dose-dependent manner with a maximum inhibition of ~40% at a concentration of 0.75 μg/ml.
Ho, D. T., Wykoff-Clary, S., Gross, C. S., Schneider, D., Jin, F., Kretschmer, P. J., & Hermiston, T. W. (2009). Growth inhibition of an established A431 xenograft tumor by a full-length anti-EGFR antibody following gene delivery by AAV. Cancer gene therapy, 16(2), 184-194.
Figure 3 Expression of 14E1 and 14E1A antibody in mouse serum and hind limb muscle following delivery by adeno-associated virus (AAV) 2/1.
(a) Athymic nude mice were given a single, bilateral intramuscular (i.m.) injection of AAV2/1-14E1 or AAV2/1-14E1A into the tibialis and gastrocnemius muscle. Tibialis muscle sections were prepared 84 days after injection and examined by immunohistochemical staining for Etag.Magnification,×200. (b) Western blot analysis of whole-cell lysates prepared from the tibialis muscle of nude mice injected with AAV2/1-14E1 or AAV2/1-14E1A. (c) AAV2/1 14E1Etag-pZAC was injected i.m. into mice, into both the tibialis and gastrocnemius of each hind limb of each animal, with a total dosage of 1×1011 genome content (GC) per mouse. (d) Either AAV2/1-14E1 or AAV2/1-14E1A was injected i,m. into mice, into both the tibialis and gastrocnemius of each hind limb of each animal, with a total dosage of 1×1011 GC per mouse.
Ho, D. T., Wykoff-Clary, S., Gross, C. S., Schneider, D., Jin, F., Kretschmer, P. J., & Hermiston, T. W. (2009). Growth inhibition of an established A431 xenograft tumor by a full-length anti-EGFR antibody following gene delivery by AAV. Cancer gene therapy, 16(2), 184-194.
Figure 4 Adeno-associated virus (AAV) 2/1-mediated delivery of 14E1 inhibits the growth of early-stage tumors and prolongs survival.
A, No Etag staining could be detected in the tumor only sample. B, AAV2/1-14E1 was injected intramuscularly into athymic nude mice at different time points relative to A431 tumor challengeC, As shown by the day -28 group, high serum levels of the 14E1 antibody at the time of tumor injection significantly prevented the enlargement of tumors. D, The significant inhibition of tumor growth resulted in dramatically improved survival time for all three 14E1 treatment groups.
Ho, D. T., Wykoff-Clary, S., Gross, C. S., Schneider, D., Jin, F., Kretschmer, P. J., & Hermiston, T. W. (2009). Growth inhibition of an established A431 xenograft tumor by a full-length anti-EGFR antibody following gene delivery by AAV. Cancer gene therapy, 16(2), 184-194.
Figure 5 Adeno-associated virus (AAV) 2/1-mediated delivery of 14E1 inhibits the growth of well-established tumors and prolongs survival.
The positive control day -7 group significantly prevented tumor growth. In both the days +13 and +20 groups, tumor growth was significantly inhibited by treatment with the AAV2/1-14E1 by day 43 and resulted in significantly enhanced survival versus the tumor alone group (P<0.03) or the ablated control group (P<0.05) by day 56.
Ho, D. T., Wykoff-Clary, S., Gross, C. S., Schneider, D., Jin, F., Kretschmer, P. J., & Hermiston, T. W. (2009). Growth inhibition of an established A431 xenograft tumor by a full-length anti-EGFR antibody following gene delivery by AAV. Cancer gene therapy, 16(2), 184-194.
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CAT | Product Name | Application | Type |
---|---|---|---|
TAB-040 | Anti-Human EGFR Recombinant Antibody (Zalutumumab) | ELISA, FC, IP, FuncS, IF, Neut, ICC | IgG1 - kappa |
TAB-119 | Anti-Human EGFR Recombinant Antibody (Necitumumab) | FC, IP, ELISA, Neut, FuncS, IF, WB | IgG1 - kappa |
TAB-278MZ-F(E) | Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-278MZ-F(E)) | ELISA, WB | Human Fab |
PABX-053 | Recombinant Human Anti-EGFR Antibody (DL11) | WB, ELISA, FuncS | IgG |
PABX-053-F (E) | Recombinant Human Anti-EGFR Antibody Fab Fragment (DL11) | WB, ELISA, FuncS | Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-003 | Anti-Human EGFR Recombinant Antibody (Cetuximab) | IF, IP, Neut, FuncS, ELISA, FC, ICC | IgG1 - kappa |
TAB-272MZ | Human Anti-EGFR Recombinant Antibody (TAB-272MZ) | FuncS | Chimeric (mouse/human) IgG |
TAB-302MZ | Human Anti-EGFR Recombinant Antibody (TAB-302MZ) | ELISA | Chimeric (mouse/human) IgG1 |
TAB-302MZ-S(P) | Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-302MZ-S(P)) | ELISA | Mouse scFv |
TAB-308MZ-S(P) | Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-308MZ-S(P)) | ELISA, FC | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
PABL-080 | Human Anti-EGFR Recombinant Antibody (PABL-080) | ELISA, WB, FuncS | Human IgG |
PABZ-039 | Mouse Anti-EGFR Recombinant Antibody (clone mAb528) | FC | Mouse IgG |
PABW-039 | Human Anti-EGFR Recombinant Antibody (clone Cetuximab (IMC-C225)) | FuncS | Chimeric (mouse/human) IgG |
PABC-040 | Human Anti-EGFR Recombinant Antibody (clone MR1) | Block | Human IgG |
FAMAB-0094YC | Human Anti-EGFR Recombinant Antibody (clone F4) | ELISA, WB, FC | Human IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
PFBZ-039 | Mouse Anti-EGFR Recombinant Antibody (clone mAb528); Fab Fragment | FC | Mouse Fab |
PFBW-039 | Human Anti-EGFR Recombinant Antibody (clone Cetuximab (IMC-C225)); Fab Fragment | FuncS | Chimeric (mouse/human) Fab |
PFBC-040 | Human Anti-EGFR Recombinant Antibody (clone MR1); Fab Fragment | Block | Human Fab |
HPAB-0010-YC-F(E) | Mouse Anti-EGFR Recombinant Antibody (clone L211C); Fab Fragment | ELISA, FC | Mouse Fab |
HPAB-0011-YC-F(E) | Human Anti-EGFR Recombinant Antibody (clone Pep 1); Fab Fragment | FC | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-0564CL-S(P) | Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-0564CL-S(P)) | ELISA | Mouse scFv |
TAB-0565CL-S(P) | Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-0565CL-S(P)) | ELISA | Mouse scFv |
TAB-310MZ-F(E) | Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-310MZ-F(E)) | ELISA | Mouse Fab |
TAB-311MZ-F(E) | Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-311MZ-F(E)) | ELISA | Mouse Fab |
TAB-312MZ-F(E) | Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-312MZ-F(E)) | ELISA | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-274MZ | Human Anti-EGFR Recombinant Antibody (TAB-274MZ) | FC | Humanized IgG |
TAB-297MZ | Anti-Human EGFR Recombinant Antibody (H225) | ELISA, WB | Humanized antibody |
TAB-274MZ-S(P) | Human Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-274MZ-S(P)) | FC | Humanized scFv |
TAB-297MZ-S(P) | Anti-Human EGFR Recombinant Antibody scFv Fragment (H225) | ELISA, WB | Humanized antibody |
TAB-298MZ-S(P) | Anti-Human EGFR Recombinant Antibody scFv Fragment (Hu225) | ELISA, WB | Humanized antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
Gly-055LC | Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated) | ELISA | Chimeric antibody (mouse/human) |
Gly-144LC | Recombinant Anti-Human EGFR Antibody (Fc glycosylation) | ELISA | Humanized antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
Gly-055LC-1 | Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated) | ELISA | Chimeric antibody (mouse/human) |
CAT | Product Name | Application | Type |
---|---|---|---|
Gly-167LC | Recombinant Anti-Human EGFR Antibody (Non-glycosylated) | ELISA | Human antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
BRD-0183MZ | Chicken Anti-EGFR Polyclonal IgY | WB | Chicken antibody |
BRD-0668MZ | Chicken Anti-EGFR Polyclonal IgY | WB | Chicken antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
MHC-LC773 | A*0201/Human EGFR (YLNTVQPTCV) MHC Tetramer | FCM | |
MHC-LC4545 | PE-DQB1*03:02/Human EGFR (SRALEEKKGNYVVTHG) MHC Tetramer | FCM | |
MHC-YF409 | A*02:01/Human EGF-R-479 (KLFGTSGQKT) MHC Pentamer | FCM |
CAT | Product Name | Application | Type |
---|---|---|---|
NEUT-722CQ | Rabbit Anti-EGFR Recombinant Antibody (clone CBL1011) | Neut | Rabbit IgG |
NEUT-723CQ | Mouse Anti-EGFR Recombinant Antibody (clone CBL931) | WB, IP, IHC, ICC, Neut | Mouse IgG1 |
NEUT-724CQ | Rabbit Anti-EGFR Recombinant Antibody (clone D1D4J) | IF, FC, WB, IP, Neut | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-1101 | Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone DS1101AB) | IHC-P | Rabbit IgG |
MOR-4520 | Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH28DS) | IF, ICC, FC | Rabbit IgG |
MOR-4675 | Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH189DS) | WB, IF, ICC, FC | Rabbit IgG |
MOR-4676 | Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH190DS) | WB, IF, ICC, FC | Rabbit IgG |
MOR-0033-FY | Rabbit Anti-EGFR Recombinant Antibody (clone AFY0004) | ICC, IHC, WB | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
AFC-TAB-165 | Afuco™ Anti-EGFR ADCC Recombinant Antibody (Matuzumab), ADCC Enhanced | Neut, ELISA, IF, IP, FuncS, FC | ADCC enhanced antibody |
AFC-TAB-464CQ | Afuco™ Anti-EGFR ADCC Recombinant Antibody (Tomuzotuximab), ADCC Enhanced | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
AFC-TAB-003 | Afuco™ Anti-EGFR ADCC Recombinant Antibody (Cetuximab), ADCC Enhanced | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
AFC-TAB-040 | Afuco™ Anti-EGFR ADCC Recombinant Antibody (Zalutumumab), ADCC Enhanced | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
AFC-TAB-119 | Afuco™ Anti-EGFR ADCC Recombinant Antibody (Necitumumab), ADCC Enhanced | FC, IP, ELISA, Neut, FuncS, IF | ADCC enhanced antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-AP881-YC | Recombinant Llama Anti-EGFR Single Domain Antibody (VHH122) | ELISA, FC, IP, FuncS | Llama VHH |
HPAB-AP882-YC | Recombinant Llama Anti-EGFR Single Domain Antibody (VHH205) | ELISA | Llama VHH |
HPAB-AP883-YC | Recombinant Llama Anti-EGFR Single Domain Antibody (VHH03) | ELISA | Llama VHH |
HPAB-AP884-YC | Recombinant Llama Anti-EGFR Single Domain Antibody (VHH139) | ELISA | Llama VHH |
HPAB-AP885-YC | Recombinant Llama Anti-EGFR Single Domain Antibody (VHH107) | ELISA | Llama VHH |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-AP637-YC-S(P) | Human Anti-EGFR Recombinant Antibody (clone EGL2); scFv Fragment | ELISA, FC, IF, Inhib | Human scFv |
HPAB-AP638-YC-S(P) | Human Anti-EGFR Recombinant Antibody (clone EGL3); scFv Fragment | ELISA, FC, IF, Inhib | Human scFv |
HPAB-2059-FY-S(P) | Mouse Anti-EGFR Recombinant Antibody (clone L2-12B); scFv Fragment | FC, ELISA | Mouse scFv |
HPAB-2060-FY-S(P) | Mouse Anti-EGFR Recombinant Antibody (clone L3-11D); scFv Fragment | FC, ELISA | Mouse scFv |
HPAB-2098-FY-S(P) | Mouse Anti-EGFR Recombinant Antibody (clone Mint5); scFv Fragment | ICC, Inhib | Mouse scFv |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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