Rabbit Anti-EGFR Recombinant Antibody (clone 5D4) (CAT#: ZG-0645J)

Figure 1 Rabbit Anti-EGFR Antibody (ZG-0645J) in WB

Western Blot
Positive WB detected in: Hela whole cell lysate, L02 whole cell lysate, HepG2 whole cell lysate
All lanes: EGFR antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 135, 45, 78, 70 kDa
Observed band size: 150 kDa

Figure 2 Rabbit Anti-EGFR Antibody (ZG-0645J) in IHC

IHC image of this product diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Figure 3 Rabbit Anti-EGFR Antibody (ZG-0645J) in IHC

IHC image of this product diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.