Rabbit Anti-ELK1 Recombinant Antibody (VS3-CJ747) (CAT#: VS3-CJ747)
This product is a rabbit antibody that recognizes human, mouse, and rat ELK1.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36084/if_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36084/if_selected.jpg
Figure 2 IF staining of human cell line SiHa
(Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64381/1164_E3_1_selected.jpg
(Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64381/1164_E3_1_selected.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3808/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3808/if_selected.jpg
Figure 4 IHC staining of human placenta
(Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3808/ihc_selected.jpg
(Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3808/ihc_selected.jpg
Figure 5 Testis
(Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Peritubular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36084/122841_A_4_6.jpg
(Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Peritubular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36084/122841_A_4_6.jpg
Figure 6 Ovary
(Ovarian stroma cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36084/122841_A_5_7.jpg
(Ovarian stroma cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36084/122841_A_5_7.jpg
Figure 7 Cerebral cortex
(Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36084/122841_B_9_5.jpg
(Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/36084/122841_B_9_5.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000126767-ELK1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000126767-ELK1
Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat ELK1
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IP
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunoprecipitation.
Target
- Alternative Names
- ETS transcription factor ELK1
- Gene ID
- 2002
- UniProt ID
- P19419
- Sequence Similarities
- Belongs to the ETS family.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.
On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Also phosphorylated on Ser-383 by MAPK8 and/or MAKP9. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity. Phosphorylated and activated by CAMK4, MAPK11, MAPK12 and MAPK14. Upon bFGF stimulus, phosphorylated by PAK1 (By similarity).
- Protein Refseq
- NP_001107595.1; NP_001244097.1; NP_005220.2
- Function
- Transcription factor that binds to purine-rich DNA sequences. Forms a ternary complex with SRF and the ETS and SRF motifs of the serum response element (SRE) on the promoter region of immediate early genes such as FOS and IER2. Induces target gene transcription upon JNK-signaling pathway stimulation (By similarity).
Recommended Products
Isotype Control
Secondary Antibody
Related Resources
cAMP Signaling Pathway
ErbB Signaling Pathway
MAPK Signaling Pathway
Ras Signaling Pathway
Colorectal Cancer
Endometrial Cancer
Gastric Cancer
Non-small Cell Lung Cancer
Throid Cancer
Insulin Resistance
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "ELK1"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0642CT | Recombinant Mouse anti-Human ELK1 Monoclonal antibody (8F20E6) | ELISA, WB | |
| MRO-0530-CN | Rabbit Anti-ELK1 Recombinant Antibody (clone CBACN-212) | WB, IF, IP | Rabbit IgG |
| ZG-0286J | Mouse Anti-ELK1 Recombinant Antibody (ZG-0286J) | WB, ELISA | Mouse IgG |
| VS3-FY493 | Recombinant Rabbit Anti-ELK1 Antibody (clone R06-5J2) | WB, IHC-P, IP, ChIP | Rabbit IgG |
| VS3-FY494 | Recombinant Rabbit Anti-ELK1 Antibody (clone R08-6K4) | WB | Rabbit IgG |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1138 | Hi-Affi™ Rabbit Anti-ELK1 Recombinant Antibody (clone DS1138AB) | ICC, IHC-P, IP, WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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