Recombinant Rabbit Anti-ELK1 Antibody (clone R06-5J2) (CAT#: VS3-FY493)

• Immunogen
• A synthetic peptide of human ELK1

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human ELK1

• Species Reactivity
• Human

• Clone
• R06-5J2

• Applications
• Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Chromatin Immunoprecipitation

• Conjugate
• Unconjugated

• MW
• Calculated MW: 45 kDa; Observed MW: 62 kDa

• Purification
• Affinity Purified

• Purity
• >95%

• Format
• Liquid

• Buffer
• 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA

• Preservative
• 0.01% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunohistochemistry: 1/50-1/100
  Immunoprecipitation: 1/20
  Chimmunoprecipitation: 1/20

• Alternative Names
• ELK1, ETS Transcription Factor; ELK1, Member Of ETS Oncogene Family

• Gene ID
• 2002

• UniProt ID
• P19419

• Sequence Similarities
• Belongs to the ETS family.

• Cellular Localization
• Nucleus

• Post Translation Modifications
• Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.
  On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Also phosphorylated on Ser-383 by MAPK8 and/or MAKP9. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity. Phosphorylated and activated by CAMK4, MAPK11, MAPK12 and MAPK14. Upon bFGF stimulus, phosphorylated by PAK1 (By similarity).

• Protein Refseq
• NP_001107595.1; NP_001244097.1; NP_005220.2

• Function
• Transcription factor that binds to purine-rich DNA sequences. Forms a ternary complex with SRF and the ETS and SRF motifs of the serum response element (SRE) on the promoter region of immediate early genes such as FOS and IER2. Induces target gene transcription upon JNK-signaling pathway stimulation (By similarity).

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody

• 2ND-057CQ
• Donkey Anti-Rabbit IgG (H&L) Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-060CQ
• Llama Anti-Rabbit IgG (H&L) Antibody

• 2ND-061CQ
• Sheep Anti-Rabbit IgG (H&L) Antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody