This product is a recombinant human antibody that recognizes FLT3. D4-3 was shown to selectively bind to human FLT3 with high affinity and block FL binding.
Figure 1 EB10, D4-3, and A2IN bind to recombinant FLT3-Fc protein and FLT3 expressed on leukemia cells.
All the three anti-FLT3 IgG also bound to surface-expressed wild-type receptor in EOL-1 and EM3 cells, as well as receptor with ITD mutations in BaF3/FLT3-ITD and MV4-11 cells, as analyzed by FACS.
Williams, B., Atkins, A., Zhang, H., Lu, D., Jimenez, X., Li, H.,... & Li, Y. (2005). Cell-based selection of internalizing fully human antagonistic antibodies directed against FLT3 for suppression of leukemia cell growth. Leukemia, 19(8), 1432.
Figure 2 The ability of purified Fab fragments and full-length IgG to competitively block FLT3/FL interaction was tested in an ELISA.
EB10, D4-3, and A2IN block FLT3/FL interaction. Serial dilutions of EB10, A2IN, or D4-3 Fab fragments (a) or IgG (b) were mixed with a fixed amount of FLT3-Fc and incubated at RT for 1 h. The mixtures were transferred to an FL-coated plate and incubated for 1 h.
Williams, B., Atkins, A., Zhang, H., Lu, D., Jimenez, X., Li, H.,... & Li, Y. (2005). Cell-based selection of internalizing fully human antagonistic antibodies directed against FLT3 for suppression of leukemia cell growth. Leukemia, 19(8), 1432.
Figure 3 Anti-FLT3 antibodies inhibit FL-induced phosphorylation of FLT3 in leukemia cells.
Treatment with FL results in strong FLT3 receptor phosphorylation in EOL-1 cells, which was significantly (85–90%) inhibited by D4-3 and EB10.
Williams, B., Atkins, A., Zhang, H., Lu, D., Jimenez, X., Li, H.,... & Li, Y. (2005). Cell-based selection of internalizing fully human antagonistic antibodies directed against FLT3 for suppression of leukemia cell growth. Leukemia, 19(8), 1432.
Figure 4 Inhibition of FL-stimulated proliferation of EOL-1 cells by the anti-FLT3 antibodies.
Cells were cultured in AIM-V medium for 68 h, with varying concentrations of antibodies (from 0 to 400 nM) in the presence of exogenous FL (30 ng/ml). In a background control, EOL-1cells were incubated with medium alone in the absence of exogenous FL. Cells were pulsed for 4 h with 3H-thymidine, harvested, and cpm measured by a beta scintillation counter. The percent of FL-induced EOL-1 cell growth inhibition was calculated using the following formula: [1-(cpm of antibody-treated sample−cpm of background sample)/(cpm of FL-only treated sample−cpm of background sample)]100%. Data shown are the mean±s.d. of triplicate determinations.
Williams, B., Atkins, A., Zhang, H., Lu, D., Jimenez, X., Li, H.,... & Li, Y. (2005). Cell-based selection of internalizing fully human antagonistic antibodies directed against FLT3 for suppression of leukemia cell growth. Leukemia, 19(8), 1432.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1630z | Mouse Anti-FLT3 Recombinant Antibody (clone 23F1) | WB, ELISA, FC, ICC, IHC | Mouse IgG1 |
MOB-0606CT | Recombinant Mouse anti-Human FLT3 Monoclonal antibody (CW20B5) | FC, IP | |
HPAB-0103-YC | Human Anti-FLT3 Recombinant Antibody (HPAB-0103-YC) | ELISA, Block, FC | Humanized IgG1, κ |
HPAB-0183CQ | Human Anti-FLT3 Recombinant Antibody (clone NC7) | ELISA, Neut | Human IgG1, κ |
VS3-CJ918 | Mouse Anti-FLT3 Recombinant Antibody (clone BV10-A4H2) | IF, FC | Mouse IgG1, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-291CL | Anti-Human FLT3 Recombinant Antibody (IMC-EB10) | WB, IHC, FC, Cyt, ELISA | Antibody |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-010CQ | Anti-Human FLT3 Recombinant Antibody (BV10A4H2) | FC | |
TAB-011CQ | Mouse Anti-FLT3 Recombinant Antibody (TAB-011CQ) | ELISA, FC | Mouse IgG1, κ |
TAB-014CQ | Mouse Anti-FLT3 Recombinant Antibody (TAB-014CQ) | ELISA, FC | Mouse IgG1, κ |
TAB-010CQ-F(E) | Anti-Human FLT3 Recombinant Antibody Fab Fragment (BV10A4H2) | ELISA, FC | |
TAB-011CQ-F(E) | Mouse Anti-FLT3 Recombinant Antibody; Fab Fragment (TAB-011CQ-F(E)) | ELISA, FC | Mouse Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
TAB-012CQ | Human Anti-FLT3 Recombinant Antibody (TAB-012CQ) | ELISA, FC | Chimeric (Mouse/Human) IgG1, κ |
TAB-012CQ-S(P) | Human Anti-FLT3 Recombinant Antibody; scFv Fragment (TAB-012CQ-S(P)) | ELISA, FC | Human scFv |
TAB-013CQ-S(P) | Human Anti-FLT3 Recombinant Antibody; scFv Fragment (TAB-013CQ-S(P)) | ELISA, FC | Human scFv |
TAB-012CQ-F(E) | Human Anti-FLT3 Recombinant Antibody; Fab Fragment (TAB-012CQ-F(E)) | ELISA, FC | Chimeric (Mouse/Human) Fab |
TAB-013CQ-F(E) | Human Anti-FLT3 Recombinant Antibody; Fab Fragment (TAB-013CQ-F(E)) | ELISA, FC | Chimeric (Mouse/Human) Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-1323 | Hi-Affi™ Rabbit Anti-FLT3 Recombinant Antibody (clone DS1323AB) | WB | Rabbit IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0103-YC-S(P) | Human Anti-FLT3 Recombinant Antibody; scFv Fragment (HPAB-0103-YC-S(P)) | ELISA, Block, FC | Human scFv |
HPAB-0182CQ-S(P) | Human Anti-FLT3 Recombinant Antibody (clone D4-3); scFv Fragment | ELISA, Neut | Human scFv |
HPAB-0183CQ-S(P) | Human Anti-FLT3 Recombinant Antibody (clone NC7); scFv Fragment | ELISA, Neut | Human scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0103-YC-F(E) | Human Anti-FLT3 Recombinant Antibody; Fab Fragment (HPAB-0103-YC-F(E)) | ELISA, Block, FC | Humanized Fab |
HPAB-0182CQ-F(E) | Human Anti-FLT3 Recombinant Antibody (clone D4-3); Fab Fragment | ELISA, Neut | Human Fab |
HPAB-0183CQ-F(E) | Human Anti-FLT3 Recombinant Antibody (clone NC7); Fab Fragment | ELISA, Neut | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
MHC-YF438 | A*01:01/Human FLT3 ITD (YVDFREYEYY) MHC Pentamer | FCM |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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