Mouse Anti-HSPD1 Recombinant Antibody (VS3-CJ576) (CAT#: VS3-CJ576)
This product is a mouse antibody that recognizes human, mouse, rat, and Monkey HSPD1.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/if_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/50025/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/50025/if_selected.jpg
Figure 3 IHC staining of human kidney
(Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells of renal tubules.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/ihc_selected.jpg
(Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells of renal tubules.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/ihc_selected.jpg
Figure 4 IHC staining of human rectum
(Immunohistochemical staining of human rectum shows strong cytoplasmic positivity, with a granular pattern, in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2775/ihc_selected.jpg
(Immunohistochemical staining of human rectum shows strong cytoplasmic positivity, with a granular pattern, in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2775/ihc_selected.jpg
Figure 5 IHC staining of human colon
(Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72816/ihc_selected.jpg
(Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/72816/ihc_selected.jpg
Figure 6 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/29_C6_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/29_C6_1_blue_red_green.jpg
Figure 7 Cerebral cortex
(Glial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_B_8_5.jpg
(Glial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_B_8_5.jpg
Figure 8 Colon
(Endocrine cells
Staining: High
Intensity: Strong
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
Mucosal lymphoid cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_7_3.jpg
(Endocrine cells
Staining: High
Intensity: Strong
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
Mucosal lymphoid cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_7_3.jpg
Figure 9 Liver
(Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_9_4.jpg
(Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_9_4.jpg
Figure 10 Pancreas
(Exocrine glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Pancreatic endocrine cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_1_3.jpg
(Exocrine glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Pancreatic endocrine cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_1_3.jpg
Figure 11 Kidney
(Bowman's capsule
Staining: High
Intensity: Strong
Quantity:>75%
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Collecting ducts
Staining: High
Intensity: Strong
Quantity:>75%
Distal tubules
Staining: High
Intensity: Strong
Quantity:>75%
Proximal tubules (cell body)
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_8_5.jpg
(Bowman's capsule
Staining: High
Intensity: Strong
Quantity:>75%
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Collecting ducts
Staining: High
Intensity: Strong
Quantity:>75%
Distal tubules
Staining: High
Intensity: Strong
Quantity:>75%
Proximal tubules (cell body)
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_8_5.jpg
Figure 12 Testis
(Elongated or late spermatids
Staining: Medium
Intensity: Moderate
Quantity:>75%
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Pachytene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Round or early spermatids
Staining: Medium
Intensity: Moderate
Quantity:>75%
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_6_6.jpg
(Elongated or late spermatids
Staining: Medium
Intensity: Moderate
Quantity:>75%
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Pachytene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Round or early spermatids
Staining: Medium
Intensity: Moderate
Quantity:>75%
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1523/6960_A_6_6.jpg
Figure 13 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000144381-HSPD1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000144381-HSPD1
Figure 13 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000144381-HSPD1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000144381-HSPD1
Specifications
- Immunogen
- Recombinant protein corresponding to full length CPN60
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human, Mouse, Rat, Monkey HSPD1
- Species Reactivity
- Human, Mouse, Rat, Monkey
- Applications
- ELISA, WB
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- 1 mg/mL (lot specific)
- Buffer
- Glycine-tris-HCl buffer, pH 7.0.
- Preservative
- 0.02% Proclin 300
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Western Blot.
Target
- Alternative Names
- HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
- Gene ID
- 3329
- UniProt ID
- P10809
- Sequence Similarities
- Belongs to the chaperonin (HSP60) family.
- Cellular Localization
- Mitochondrion
- Protein Refseq
- NP_002147.2; NP_955472.1
- Function
- Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix (PubMed:1346131, PubMed:11422376).
The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein (Probable).
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "HSPD1"
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-1035 | Mouse Anti-HSPD1 Recombinant Antibody (clone YC181); Fab Fragment | ELISA | Mouse Fab |
| FAMAB-0119JF-F(E) | Mouse Anti-HSPD1 Recombinant Antibody (clone II-13); Fab Fragment | WB, ELISA, FuncS | Mouse Fab |
| FAMAB-0120JF-F(E) | Mouse Anti-HSPD1 Recombinant Antibody (clone ML-30); Fab Fragment | WB, ELISA, FuncS | Mouse Fab |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-110CT | Anti-Human HSP60 Recombinant Antibody (prozumab) | Inhibit, ELISA | Humanized antibody |
| TAB-110CT-S(P) | Anti-Human HSP60 Recombinant Antibody scFv Fragment (prozumab) | Inhibit, ELISA | Humanized antibody |
| TAB-110CT-F(E) | Anti-Human HSP60 Recombinant Antibody Fab Fragment (prozumab) | Inhibit, ELISA | Humanized antibody |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-018CT | Recombinant Mouse Anti-HSP60 Antibody | WB, IHC, FC |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0880CT | Recombinant Mouse anti-Human HSPD1 Monoclonal antibody (2E22CE9) | FC, ICC/IF, ELISA, WB | |
| FAMAB-0119JF | Mouse Anti-HSPD1 Recombinant Antibody (clone II-13) | WB, IHC, FuncS | Mouse IgG2a |
| FAMAB-0120JF | Mouse Anti-HSPD1 Recombinant Antibody (clone ML-30) | WB, ELISA, IHC, IF | Mouse IgG1 |
| MOB-0044F | Mouse Anti-HSPD1 Recombinant Antibody (MOB-0044F) | IHC-P, IF | Mouse IgG |
| ZG-0754J | Rabbit Anti-HSPD1 Recombinant Antibody (clone 3D8) | ELISA, WB, IHC, IP | Rabbit IgG |
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-920CQ | Mouse Anti-HSPD1 Recombinant Antibody (clone 4B9/89) | Block, ELISA, ICC, IF, IHC, IHC-P, IP, WB | Mouse IgG2a |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1704 | Rabbit Anti-HSPD1 Recombinant Antibody (clone DS1704AB) | IHC-P, WB | Rabbit IgG |
| MOR-0077-FY | Rabbit Anti-HSPD1 Recombinant Antibody (clone AFY0048) | ICC, IHC-P, WB | Rabbit IgG |
MHC Tetramer for Cancer
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC2077 | PE-A*02:01/Human HSPD1 (KLSDGVAVL) MHC Tetramer | FCM |
Epitope-Specific Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| EPAF-1428LC | Mouse Anti-HSPD1 Recombinant Antibody (clone 9C9) | ELISA | Mouse IgG1 |
MHC Tetramer for Other Applications
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-YF309 | Qa-1b/Human Hsp60 (GMKFDRGYI) MHC Monomer | MHC Multimer |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0119JF-S(P) | Mouse Anti-HSPD1 Recombinant Antibody (clone II-13); scFv Fragment | WB, ELISA, FuncS | Mouse scFv |
| FAMAB-0120JF-S(P) | Mouse Anti-HSPD1 Recombinant Antibody (clone ML-30); scFv Fragment | WB, ELISA, FuncS | Mouse scFv |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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