Recombinant Mouse Anti-JEV Antibody (2F2) (CAT#: PABC-364)

Figure 1 2F2 and 2H4 neutralize JEV by inhibiting attachment and fusion of JEV.

a, The amount of virions on the cell surface was detected by RT-PCR after exposure to 2F2 or 2H4, before (left) and after (right) the virus was allowed to attach to cells. High concentrations of 2F2 and 2H4 prevented attachment of JEV to the cell surface when JEV was exposed to antibodies before cell attachment. b, 2F2 and 2H4 block low-pH-induced fusion of JEV with liposomes. The liposomes were loaded with self-quenching concentrations of the fluorescent dye calcein. Fusion of JEV with liposomes occurred at pH 5.6.

Qiu, X., Lei, Y., Yang, P., Gao, Q., Wang, N., Cao, L., ... & Ding, T. (2018). Structural basis for neutralization of Japanese encephalitis virus by two potent therapeutic antibodies. Nature microbiology, 3(3), 287.

Figure 2 Immunofluorescence analysis of S2/ZF-ZR and S2/JEF-JER cells with antibody 2F2.

S2 cells were transfected with pAc/ZF-ZR (A) or pAc/JEF-JER ( B). Transfected cells were harvested in PBS and plated onto microscopic slides. The cells were fixed in 10% formalin with 1% Triton X-100 at room temperature for 30 min. The cells were incubated subsequently with JEV E specific monoclonal antibody 2F2 and a fluorescein-conjugated secondary antibody (DAKO) at room temperature for 1 h. Each incubation was followed by two washes in PBS. Finally the slides were mounted with VECTASHIELD Mounting Medium containing propidium iodide and viewed under an Olympus fluorescence microscope.

Zhang, F., Ma, W., Zhang, L., Aasa-Chapman, M., & Zhang, H. (2007). Expression of particulate-form of Japanese encephalitis virus envelope protein in a stably transfected Drosophila cell line. Virology journal, 4(1), 17.

Figure 3 Comparison of recombinant JEV E protein expressed in deferent cells by Western blot.

Cell lysate were resolved by 12% SDS-PAGE under non-reducing conditions. After electrophoresis proteins were transferred onto nitrocellulose membrane and probed with the antibody 2F2. ECL reagents were used to visualise protein bands. Lanes 1 to 6 are the cell lysate from Vero/ZF-ZR, Vero/JEF-JER, S2/ZF-ZR, S2/JEF-JER, Vero and S2 cells respectively.

Zhang, F., Ma, W., Zhang, L., Aasa-Chapman, M., & Zhang, H. (2007). Expression of particulate-form of Japanese encephalitis virus envelope protein in a stably transfected Drosophila cell line. Virology journal, 4(1), 17.