Rabbit Anti-KDM1A Recombinant Antibody (clone R06-9E8) (CAT#: VS3-XY3404)
It is a recombinant rabbit antibody that recognizes KDM1A (Lysine demethylase 1A), which acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. The antibody recognizes and binds to human, mouse and rat KDM1A. This antibody has been used in Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation, Immunocytochemistry or Immunofluorescence.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/53660/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/53660/if_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5884/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5884/if_selected.jpg
Figure 3 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5884/15747_A_7_4.jpg
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5884/15747_A_7_4.jpg
Figure 4 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5884/15747_A_8_5.jpg
(Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5884/15747_A_8_5.jpg
Figure 5 Testis
(Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5884/15747_A_4_6.jpg
(Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5884/15747_A_4_6.jpg
Figure 6 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000004487-KDM1A
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000004487-KDM1A
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat KDM1A
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R06-9E8
- Applications
- WB, IHC-P, ICC, IF, IP
- Related Disease
- Cleft Palate; Psychomotor Retardation; Distinctive Facial Features; Neuroblastoma
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/50-1/100
IF: 1/50-1/200
IP: 1/20
Target
- Alternative Names
- AOF2; CPRF; KDM1; LSD1; BHC110
- Gene ID
- 23028
- UniProt ID
- O60341
- Long Name
- Lysine demethylase 1A
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Polyubiquitinated by JADE2; which leads to its proteasomal degradation (PubMed:25018020).
Deubiquitinated by USP38; preventing it from degradation by the 26S proteasome (PubMed:30497519).
(O60341-KDM1A_HUMAN)
Ubiquitination at Lys280, Lys322, Lys347, Lys355, Lys374, Lys492, Lys503, Lys647, Lys732, and Lys744 (NX_O60341 [NX_O60341-1])
Modification sites at PhosphoSitePlus (O60341)
Glycosylation from GlyGen (O60341) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_006710536.1
- Function
- Histone demethylase that can demethylate both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context (PubMed:15620353, 15811342, 16140033, 16079794, 16079795, 16223729).
Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed (PubMed:15620353, 15811342, 16079794, 21300290).
Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation.
Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me (PubMed:15620353, 20389281, 21300290, 23721412).
May play a role in the repression of neuronal genes.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "KDM1A"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2167MZ | Recombinant Mouse Anti-Human KDM1A Antibody | ELISA, ELISA, ICC/IF, IP, WB | Mouse antibody |
| MRO-2018-CN | Rabbit Anti-KDM1A Polyclonal Antibody (MRO-2018-CN) | WB, IF, IHC, FC | Rabbit IgG |
| ZG-0784J | Rabbit Anti-KDM1A Recombinant Antibody (clone 1G7) | ELISA, WB, IHC | Rabbit IgG |
| VS3-XY960 | Mouse Anti-KDM1A Recombinant Antibody (clone 3G1) | WB, IP, IF | Mouse IgG1 |
| VS3-FY1755 | Rabbit Anti-KDM1A Recombinant Antibody (clone R08-3J5) | WB, IF, IP | Rabbit IgG |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1924 | Hi-Affi™ Recombinant Rabbit Anti-KDM1A Monoclonal Antibody (DS1924AB) | WB, IHC, ICC, IP | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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