Rabbit Anti-NKX2-1 Recombinant Antibody (clone R09-1H7) (CAT#: VS3-FY1918)
This R09-1H7 recombinant monoclonal antibody is specifically designed to target human NKX2-1. It cross-reacts with hamster. It is suitable for use in a variety of assays, including WB, ICC/IF. The antibody has the positive detection of thyroid transcription factor 1 protein by western blot analysis of TSG101 in K562 lysates and immunocytochemistry analysis of thyroid transcription factor 1 (green) in K562 using thyroid transcription factor 1 antibody.
Specific Inquiry
WB
Figure 1 Anti-NKX2-1 Antibody (VS3-FY1918) in WB.
The TTF1 expression in K562 lysates were detected using the anti-TTF1 by western blot assay.
ICC
Figure 2 Anti-NKX2-1 Antibody (VS3-FY1918) in ICC.
Immunocytochemistry staining of Thyroid Transcription Factor 1 (green) in K562 using the anti-Thyroid Transcription Factor 1 antibody. Nuclear DNA was labeled with DAPI (shown in blue).
Specifications
- Immunogen
- Synthetic peptide of human TTF1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Hamster NKX2-1
- Species Reactivity
- Human, Hamster
- Clone
- R09-1H7
- Applications
- Western Blot, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 39 kDa, while the observed molecular weight stands at 39 kDa.
- Related Disease
- Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- NK2 Homeobox 1; Thyroid Transcription Factor 1; Thyroid-Specific Enhancer-Binding Protein; Homeobox Protein NK-2 Homolog A; Thyroid Nuclear Factor 1; NKX2A; TITF1; T/EBP; TTF-1; TTF1
- Gene ID
- 7080
- UniProt ID
- P43699
- Sequence Similarities
- Belongs to the NK-2 homeobox family.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Phosphorylated on serine residues by STK3/MST2. (P43699-NKX21_HUMAN)
Modification sites at PhosphoSitePlus (P43699)
Modification sites at neXtProt (NX_P43699)
Glycosylation from GlyGen (P43699) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001073136.1; NP_003308.1
- Function
- May play a role in lung development and surfactant homeostasis. Forms a regulatory loop with GRHL2 that coordinates lung epithelial cell morphogenesis and differentiation. Activates the transcription of GNRHR and plays a role in enhancing the circadian oscillation of its gene expression.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "NKX2-1"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1310z | Mouse Anti-NKX2-1 Recombinant Antibody (clone 11B10) | IF, IHC, WB | Mouse IgG2b |
| MOB-0342MZ | Recombinant Mouse Anti-Human NK2 Homeobox 1 Antibody (clone 9H8G4/2) | IHC | Mouse antibody |
| ZG-0487U | Rabbit Anti-NKX2-1 Recombinant Antibody (clone 6C11) | ELISA, WB, IHC, FC | Rabbit IgG |
| VS3-FY1045 | Recombinant Rabbit Anti-NKX2-1 Antibody (clone R02-6A5) | WB, IHC-P, IP | Rabbit IgG |
| VS3-XY3446 | Rabbit Anti-NKX2-1 Recombinant Antibody (clone R07-3I9) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2449 | Hi-Affi™ Recombinant Rabbit Anti-NKX2-1 Monoclonal Antibody (DS2449AB) | IHC-P | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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