Rabbit Anti-PDGFRB Recombinant Antibody (clone R02-5B2) (CAT#: VS3-FY1636)
The R02-5B2 recombinant monoclonal antibody has the ability to specifically bind to human PDGFRB protein, a calculated weight of 124 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R02-5B2 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunoprecipitation (dilution range 1:20).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line ASC TERT1
(Immunofluorescent staining of human cell line ASC TERT1 shows localization to the Golgi apparatus & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/28499/1774_C4_13_cr5950fa3646972_selected.jpg
(Immunofluorescent staining of human cell line ASC TERT1 shows localization to the Golgi apparatus & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/28499/1774_C4_13_cr5950fa3646972_selected.jpg
Figure 2 Testis
(Pachytene spermatocytes Staining: Low Intensity: Moderate Quantity: <25% Peritubular cells Staining: High Intensity: Strong Quantity: 75%-25% Spermatogonia cells Staining: Low Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3842/10254_A_4_6.jpg
(Pachytene spermatocytes Staining: Low Intensity: Moderate Quantity: <25% Peritubular cells Staining: High Intensity: Strong Quantity: 75%-25% Spermatogonia cells Staining: Low Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3842/10254_A_4_6.jpg
Figure 3 Placenta
(Decidual cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Trophoblastic cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3842/10254_A_2_7.jpg
(Decidual cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Trophoblastic cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3842/10254_A_2_7.jpg
Figure 4 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3842/10254_B_9_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3842/10254_B_9_5.jpg
Figure 5 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3842/10254_A_9_4.jpg
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3842/10254_A_9_4.jpg
Figure 6 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3842/10254_A_8_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3842/10254_A_8_5.jpg
Figure 7 RNA cell line category: Group enriched (ASC diff, ASC TERT1)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113721-PDGFRB
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113721-PDGFRB
Specifications
- Immunogen
- Synthetic peptide of human PDGFR beta
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat PDGFRB
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R02-5B2
- Applications
- Western Blot, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 124 kDa, while the observed molecular weight stands at 190 kDa.
- Related Disease
- Kosaki Overgrowth Syndrome and Premature Aging Syndrome, Penttinen Type
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunoprecipitation: 1/20
Target
- Alternative Names
- Platelet Derived Growth Factor Receptor Beta; Platelet-Derived Growth Factor Receptor, Beta Polypeptide; Beta-Type Platelet-Derived Growth Factor Receptor; Platelet-Derived Growth Factor Receptor 1; CD140 Antigen-Like Family Member B; PDGF-R-Beta; EC 2.7.10.1; PDGFR-Beta; PDGFR-1; PDGFR1; PDGFR; Platelet-Derived Growth Factor Receptor Beta
- Gene ID
- 5159
- UniProt ID
- P09619
- Sequence Similarities
- Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
- Cellular Localization
- Cell membrane, Cytoplasmic vesicle, Lysosome, Membrane
- Post Translation Modifications
- Disulfide bond, Glycoprotein, Phosphoprotein
- Protein Refseq
- NP_001341945.1; NP_001341946.1; NP_002600.1
- Function
- Tyrosine-protein kinase that acts as cell-surface receptor for homodimeric PDGFB and PDGFD and for heterodimers formed by PDGFA and PDGFB, and plays an essential role in the regulation of embryonic development, cell proliferation, survival, differentiation, chemotaxis and migration. Plays an essential role in blood vessel development by promoting proliferation, migration and recruitment of pericytes and smooth muscle cells to endothelial cells.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "PDGFRB"
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-706CL | Anti-Human PDGFRB Therapeutical Antibody (IMC-2C5) | Inhib, FuncS | Antibody |
| TAB-0988CL-F(E) | Human Anti-PDGFRB Recombinant Antibody; Fab Fragment (TAB-0988CL-F(E)) | ELISA | Human Fab |
| TAB-0989CL-F(E) | Human Anti-PDGFRB Recombinant Antibody; Fab Fragment (TAB-0989CL-F(E)) | ELISA | Human Fab |
| TAB-0990CL-F(E) | Human Anti-PDGFRB Recombinant Antibody; Fab Fragment (TAB-0990CL-F(E)) | ELISA | Human Fab |
| TAB-0991CL-F(E) | Human Anti-PDGFRB Recombinant Antibody (TAB-0991CL-F(E)); Fab Fragment | ELISA | Human Fab |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1850CQ | Recombinant Rat Anti-Pdgfrb Antibody (APB5) | FC, FuncS, Neut | IgG2a, κ |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2638 | Hi-Affi™ Recombinant Rabbit Anti-PDGFRB Monoclonal Antibody (DS2638AB) | WB | IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0281CQ | Human Anti-PDGFRB Recombinant Antibody (clone 1B3) | ELISA, FC, WB, Neut | Human IgG |
| MRO-1193-CN | Recombinant Rabbit Anti-PDGFRB Monoclonal Antibody (CBACN-438) | WB, IHC, IP | Rabbit IgG |
| HPAB-1110-CN | Mouse Anti-PDGFRB Recombinant Antibody (HPAB-1110-CN) | ELISA | Mouse IgG1 |
| HPAB-1111-CN | Human Anti-PDGFRB Recombinant Antibody (HPAB-1111-CN) | ELISA | Human IgG4 |
| HPAB-1112-CN | Human Anti-PDGFRB Recombinant Antibody (HPAB-1112-CN) | ELISA | Human IgG4 |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0281CQ-F(E) | Human Anti-PDGFRB Recombinant Antibody (clone 1B3); Fab Fragment | ELISA, FC, WB, Neut | Human Fab |
| HPAB-1110-CN-F(E) | Mouse Anti-PDGFRB Recombinant Antibody; Fab Fragment (HPAB-1110-CN-F(E)) | ELISA | Mouse Fab |
| HPAB-1111-CN-F(E) | Human Anti-PDGFRB Recombinant Antibody; Fab Fragment (HPAB-1111-CN-F(E)) | ELISA | Human Fab |
| HPAB-1112-CN-F(E) | Human Anti-PDGFRB Recombinant Antibody; Fab Fragment (HPAB-1112-CN-F(E)) | ELISA | Human Fab |
| HPAB-1113-CN-F(E) | Human Anti-PDGFRB Recombinant Antibody; Fab Fragment (HPAB-1113-CN-F(E)) | ELISA | Human Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0281CQ-S(P) | Human Anti-PDGFRB Recombinant Antibody (clone 1B3); scFv Fragment | ELISA, FC, WB, Neut | Human scFv |
| HPAB-N0227-YC-S(P) | Human Anti-PDGFRB Recombinant Antibody (clone PFH2); scFv Fragment | Block | Human scFv |
| HPAB-S0106-YC-S(P) | Human Anti-PDGFRB Recombinant Antibody (clone HuM4TS.11); scFv Fragment | ELISA, Neut | Humanized scFv |
| HPAB-S0107-YC-S(P) | Mouse Anti-PDGFRB Recombinant Antibody (clone muM4TS.22); scFv Fragment | ELISA, Neut | Mouse scFv |
| HPAB-S0108-YC-S(P) | Human Anti-PDGFRB Recombinant Antibody (clone HuM4TS.22); scFv Fragment | ELISA, Neut | Humanized scFv |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-448CQ | Afuco™ Anti-PDGFRB Recombinant Antibody (AFC-TAB-448CQ), ADCC Enhanced | ELISA, IHC, FC, IP, IF, FuncS | Human IgG1, κ |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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