Rabbit Anti-POLR2A Recombinant Antibody (clone R06-8G7) (CAT#: VS3-FY1956)

• Immunogen
• Synthetic peptide of human RNA polymerase II CTD repeat sequence YSPTSPS

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human POLR2A

• Species Reactivity
• Human

• Clone
• R06-8G7

• Applications
• Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation

• MW
• The calculated weight of the target protein is approximately 217 kDa, while the observed molecular weight stands at 250 kDa.

• Related Disease
• Neurodevelopmental Disorder With Hypotonia And Variable Intellectual And Behavioral Abnormalities and Skull Base Meningioma

• Purification
• Affinity Purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA

• Preservative
• 0.01% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunohistochemistry: 1/50-1/100
  Immunofluorescence: 1/50-1/200
  Immunoprecipitation: 1/20

• Alternative Names
• RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; NEDHIB; RpIILS; hsRPB1; hRPB220

• Gene ID
• 5430

• UniProt ID
• P24928

• Sequence Similarities
• Belongs to the RNA polymerase beta' chain family.

• Cellular Localization
• Chromosome, Cytoplasm, DNA-directed RNA polymerase, Nucleus

• Post Translation Modifications
• The tandem heptapeptide repeats in the C-terminal domain (CTD) can be highly phosphorylated (PubMed:28076779).
  The phosphorylation activates Pol II.
  Phosphorylation occurs mainly at residues 'Ser-2' and 'Ser-5' of the heptapeptide repeat and is mediated, at least, by CDK7 and CDK9.
  CDK7 phosphorylation of POLR2A associated with DNA promotes transcription initiation by triggering dissociation from DNA.
  Phosphorylation also takes place at 'Ser-7' of the heptapeptide repeat, which is required for efficient transcription of snRNA genes and processing of the transcripts.
  The phosphorylation state is believed to result from the balanced action of site-specific CTD kinases and phosphatases, and a 'CTD code' that specifies the position of Pol II within the transcription cycle has been proposed.
  Dephosphorylated by the protein phosphatase CTDSP1.
  Dephosphorylated at 'Ser-2' following UV irradiation.

• Protein Refseq
• NP_000928.1

• Function
• Pol II-mediated transcription cycle proceeds through transcription initiation, transcription elongation and transcription termination stages. During transcription initiation, Pol II pre-initiation complex (PIC) is recruited to DNA promoters, with focused-type promoters containing either the initiator (Inr) element, or the TATA-box found in cell-type specific genes and dispersed-type promoters that often contain hypomethylated CpG islands usually found in housekeeping genes. Once the polymerase has escaped from the promoter it enters the elongation phase during which RNA is actively polymerized, based on complementarity with the template DNA strand. Transcription termination involves the release of the RNA transcript and polymerase from the DNA.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody