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Mouse Anti-AKT2 Recombinant Antibody (clone 2A1) (CAT#: VS3-XY37)

Clone 2A1 is a mouse antibody, which reacts with human AKT2 protein. This antibody is suitable for AKT2 detection by WB, IP, IF, IHC assays.


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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A549
Figure 1 IF staining of human cell line A549
(Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm, cytosol & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64521/1304_E6_3_selected.jpg
Figure 2 IHC staining of human cerebellum
Figure 2 IHC staining of human cerebellum
(Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity(granular pattern) in purkinje cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4204/ihc_selected.jpg
Figure 3 IF staining of human cell line RT4
Figure 3 IF staining of human cell line RT4
(Immunofluorescent staining of human cell line RT4 shows localization to cytosol & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64521/1518_H7_2_blue_red_green.jpg
Figure 4 IF staining of human cell line U-2 OS
Figure 4 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64521/1323_E6_2_blue_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Figure 5 Cerebral cortex
(Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4204/11546_B_9_5.jpg
Figure 6 Colon
Figure 6 Colon
(Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4204/11546_A_9_3.jpg
Figure 7 Liver
Figure 7 Liver
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4204/11546_A_9_4.jpg
Figure 8 Kidney
Figure 8 Kidney
(Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4204/11546_A_7_5.jpg
Figure 9 Testis
Figure 9 Testis
(Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4204/11546_A_4_6.jpg
Figure 10 Lymph node
Figure 10 Lymph node
(Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4204/11546_A_9_8.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Figure 11 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000105221-AKT2

Specifications

  • Host Species
  • Mouse
  • Type
  • Mouse IgG1
  • Specificity
  • Human AKT2
  • Species Reactivity
  • Human
  • Clone
  • 2A1
  • Applications
  • Western Blot, Immunoprecipitation, Immunofluorescence, Immunohistochemistry

Product Property

  • Purification
  • Protein A affinity purified
  • Format
  • Liquid
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • AKT Serine/Threonine Kinase 2; V-Akt Murine Thymoma Viral Oncogene Homolog 2; Protein Kinase B Beta; Protein Kinase Akt-2; RAC-PK-Beta; EC 2.7.11.1; PKB Beta; Putative V-Akt Murine Thymoma Viral Oncoprotein 2; RAC-Beta Serine/Threonine-Protein Kinase

Related Resources

  • Related Signaling Pathways
  • Related Diseases

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "AKT2"

Single-domain Antibody

CAT Product Name Application Type
NAB-49-sdAb Recombinant Anti-human AKT2 VHH Single Domain Antibody IP, FC, ChiP, Neut Llama VHH

Recombinant Antibody

CAT Product Name Application Type
MOB-0574MZ Mouse Anti-AKT2 Recombinant Antibody (clone 95C657.1.2) IF, WB Mouse IgM
ZG-0430U Rabbit Anti-AKT2 Recombinant Antibody (clone Y14-H), FITC FC Rabbit IgG
ZG-0431U Rabbit Anti-AKT2 Recombinant Antibody (clone S7-R), FITC FC Rabbit IgG
VS3-QX35 Mouse Anti-AKT2 Recombinant Antibody (clone 148CT5.4.1.4) WB, ICC, IHC Mouse IgG1
VS3-QX36 Mouse Anti-AKT2 Recombinant Antibody (clone 7F12-H0-B7) WB, IHC, ICC, FC, ELISA Mouse IgG2b

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0033MZ Chicken Anti-AKT2 Polyclonal IgY Indirect ELISA, WB Chicken antibody

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-0128 Hi-Affi™ Rabbit Anti-AKT2 Recombinant Antibody (clone DS128AB) WB Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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