Recombinant Mouse Anti-CD3E Antibody (clone 8D6-6C1-3B2) (CAT#: VS3-FY285)

• Immunogen
• Synthesized peptide derived from human CD3

• Host Species
• Mouse

• Type
• Mouse IgG2b

• Specificity
• Human CD3E

• Species Reactivity
• Human

• Clone
• 8D6-6C1-3B2

• Applications
• Immunohistochemistry-Paraffin

• Conjugate
• Unconjugated

• Purification
• Affinity Chromatography Purified

• Purity
• >95%

• Format
• Liquid

• Buffer
• PBS pH 7.3, 50% Glycerol, 0.5% BSA

• Preservative
• 0.02% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Immunohistochemistry: 1/100-1/500

• Alternative Names
• CD3e Molecule; CD3e Antigen, Epsilon Polypeptide (TiT3 Complex); T-Cell Surface Antigen T3/Leu-4 Epsilon Chain; CD3e Molecule, Epsilon (CD3-TCR Complex); T3E; T-Cell Antigen Receptor Complex, Epsilon Subunit Of T3

• Gene ID
• 916

• UniProt ID
• P07766

• Cellular Localization
• Cell membrane, Membrane

• Post Translation Modifications
• Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.

• Protein Refseq
• NP_000724.1

• Function
• Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098).
  In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128).

Secondary Antibody

• 2ND-049CQ
• Goat Anti-Mouse IgG2b Antibody, R-PE

• 2RD-A1466-YJ
• Goat Anti-Mouse IgG2b Secondary antibody

• 2RD-A1560-YJ
• Rabbit Anti-Mouse IgG2b Secondary antibody (Clone RM108)

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS