Recombinant Mouse Anti-KRT8 Antibody (VS4-CJ14) (CAT#: VS4-CJ14)
This mouse anti-KRT8 antibody is a product that recognizes human, mouse, rat KRT8. The immunogen of this antibody is a synthetic peptide corresponding to KRT8. The mAb has been tested for use in ELISA, Western Blot (1:500-1:5000), Immunohistochemistry (1:50-1:500).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/if_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/if_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/131/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/131/if_selected.jpg
Figure 3 IHC staining of human stomach
(Immunohistochemical staining of human stomach, upper shows strong cytoplasmic and membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/ihc_selected.jpg
(Immunohistochemical staining of human stomach, upper shows strong cytoplasmic and membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/ihc_selected.jpg
Figure 4 Colon
(Endocrine cells
Staining:
High
Intensity: Strong
Enterocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Enterocytes - Microvilli
Staining:
High
Intensity: Strong
Goblet cells
Staining:
High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/117727_A_7_3.jpg
(Endocrine cells
Staining:
High
Intensity: Strong
Enterocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Enterocytes - Microvilli
Staining:
High
Intensity: Strong
Goblet cells
Staining:
High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/117727_A_7_3.jpg
Figure 5 Liver
(Cholangiocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Hepatocytes
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/117727_A_8_4.jpg
(Cholangiocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Hepatocytes
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/117727_A_8_4.jpg
Figure 6 Kidney
(Cells in tubules
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/117727_A_8_5.jpg
(Cells in tubules
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/117727_A_8_5.jpg
Figure 7 Testis
(Spermatogonia cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/117727_A_5_6.jpg
(Spermatogonia cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/117727_A_5_6.jpg
Figure 8 Prostate
(Glandular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/117727_A_3_5.jpg
(Glandular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/49866/117727_A_3_5.jpg
Figure 9 RNA cell line category: Cell line enhanced (A549, BEWO, CACO-2, CAPAN-2, MCF7, SK-BR-3, T-47d)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170421-KRT8
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170421-KRT8
Figure 9 RNA cell line category: Cell line enhanced (A549, BEWO, CACO-2, CAPAN-2, MCF7, SK-BR-3, T-47d)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170421-KRT8
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000170421-KRT8
Specifications
- Immunogen
- Synthetic Peptide
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human, Mouse, Rat KRT8
- Species Reactivity
- Human, Mouse, Rat
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Immunogen affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, PBS, pH 7.4.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at -20°C or -80°C upon receipt. Avoid repeated freeze.
Applications
- Application Notes
- This antibody has been tested for use in ELISA, Western Blot (1:500-1:5000), Immunohistochemistry (1:50-1:500).
Target
- Alternative Names
- K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
- Gene ID
- 3856
- UniProt ID
- P05787
- Long Name
- Keratin 8
- Sequence Similarities
- Belongs to the intermediate filament family.
- Cellular Localization
- Cytoplasm, Intermediate filament, Keratin, Nucleus
- Post Translation Modifications
- Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
- Protein Refseq
- NP_001243222.1; NP_002264.1
- Function
- Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "KRT8"
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1993 | Hi-Affi™ Recombinant Rabbit Anti-KRT8 Monoclonal Antibody (DS1993AB) | IHC-P, IHC-Fr | IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0452-CN | Recombinant Mouse Anti-KRT8 Monoclonal Antibody (A1-B11) | WB, IF, IHC, FC | Mouse IgG1 |
| MRO-1808-CN | Rabbit Anti-KRT8 Polyclonal Antibody (MRO-1808-CN) | WB, IF, IHC, FC | Rabbit IgG |
| MRO-1809-CN | Rabbit Anti-KRT8 Polyclonal Antibody (MRO-1809-CN) | WB, IF, IHC, FC | Rabbit IgG |
| ZG-1076F | Mouse Anti-KRT8 Recombinant Antibody (clone RCK102) | FC, ICC, IHC-Fr, IHC-P, WB | Mouse IgG1 |
| VS3-XY3413 | Rabbit Anti-KRT8 Recombinant Antibody (clone R01-9E6) | WB, IHC-P, ICC, IF, IP, FC | Rabbit IgG |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0199-WJ-S(P) | Mouse Anti-KRT8 Recombinant Antibody (clone 8G8); scFv Fragment | ELISA, IHC | Mouse scFv |
| FAMAB-0344JF-S(P) | Human Anti-KRT8 Recombinant Antibody (clone COU-1); scFv Fragment | IHC, ELISA, FuncS | Human scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0199-WJ-F(E) | Mouse Anti-KRT8 Recombinant Antibody (clone 8G8); Fab Fragment | ELISA, IHC | Mouse Fab |
| FAMAB-0344JF-F(E) | Human Anti-KRT8 Recombinant Antibody (clone COU-1); Fab Fragment | IHC, ELISA, FuncS | Human Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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